Influence of estrogen on metabolism and antioxidative functions in old cas-trated female rats

Abstract

Abstract: AIM: To investigate the influence of di-ethylstilbestrol on metabolism and antioxidative functions in old castrated female rats in order to elucidate the physi-ological and pharmacological effects of estrogen therapy in climacteric. METHODS: The old female rats had been castrated, liver drug-metabolizing enzyme activities and antioxidative indexes were measured in rats treated with andwithout diethylstilbestrol (100 μg°4kg^-1, qd×40 d, ig). RESULTS: The activities of microsomal NADPH-Cyt C reductase, benzphetamine N-demethylase, aminopyrine N-demethylase, glutathione S-transferase, and the contents of microsomal cytochrome P450, cy-tochrome b₅ and homogenate glutathione remarkable de-creased in the liver of castrated female rats. Meanwhile, an increase of liver malondialdehyde content was also ob-served. Treatment with diethylstilbestrol could reverse the changes of above indices except for cytochrome b₅. CONCLUSION: The decreased metabolism and antioxidative functions are related to the lower level of estrogen in old castrated female rats.Estrogen plays a significant role in keeping normal metabolism and antioxidative capacities of the liver.

Key words: castrated, rats, estrogen, drug-metabo-lizing function, antioxidative function

CLC Number:

R977.12

ZHANG Shao-ying, PING Jie, WANG Hui. Influence of estrogen on metabolism and antioxidative functions in old cas-trated female rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(4): 424-427.

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