Effects of huperzine A on theta-rhythm and long-term potentiation in CA1 pyramidal neurons in adult rat hippocampal slices

Abstract

Abstract: AIM: To study the effects of huperzine A on the theta-rhythm and long-term potentiation in CA1 py-ramidal neurons in adult rat hippocampal slices and to get insight into the cell electrophysiological mechanisms un-derlying the potentiation of learning and memory by Hu-perzine A.METHODS: The intracellular recordings from CA1 pyramidal neurons in hippocampal slices related to learning and memory were made to analyze the mecha-nisms of Hup-A action.RESULTS: Power of frequencies ranged from 4 to 10Hz wasnot significantly difference be-tween pre-membrane potential oscillations (MPOs) and post-MPOs in control group, but it was significantly (P<0.005, n =3) changed between pre-MPOs before ad-dition of Hup-A and post-MPOs after addition of Hup-A. During long-term potentiation (LTP), the increase of am-plitudes of EPSPs was observed at post-tetanus 30 min in control group and 15 min in Hup-A group.CONCLUSION: Hup-A can increase the power of 4 to 10 Hz MPOs and facilitate induction of LTP in CA1 pyramidal neurons of rat hippocampal slices, which may contribute to the potentiation of learning and memory by Huperzine A.

Key words: rats, hippocampal slices, pyramidal neuron, theta-rhythm, long-term potentiation (LTP), hu-perzine A

CLC Number:

R971.91

WU Xiao-wei, WANG Meng-ya. Effects of huperzine A on theta-rhythm and long-term potentiation in CA1 pyramidal neurons in adult rat hippocampal slices[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(5): 494-498.

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