Inhibition effects of U50, 488H on hypertrophic myocardial cells induced by isoproterenol

Abstract

Abstract: AIM: To observe the inhibition effects of cross-talk between kappa opioid receptor (κ-OR)and β₁ -adrenoceptor (β₁ -AR)on hypertrophic myocardial cells induced by isoproterenol (ISO)in neonatal rats.METHODS: The total protein content was assayed by the meth-od of Lowry's.The cardiomyocytes' volume was mea-sured by computer photograph analysis system and the protein synthesis was assayed with [³H] leucine intake method. RESULTS: ISO enhanced the total protein con-tent, the cardiomyocytes' volume and the protein synthe-sis in myocardial cells.U50, 488H (U50)showed the function inhibited by nor-BNI on reducing the previous mentioned indices induced by ISO.U50 also attenuated the hypertrophy induced by ISO combined with ICI118, 551(β₂ -AR inhibitor).CONCLUSION: κ-OR has a cross-talkwith β₁ -adrenoceptor in ISO-induced myocardial hypertrophy.

Key words: isoproterenol, hypertrophic myocardial cells, U50, 488H, nor-BNI, ICI18, 551

CLC Number:

R966

SHAN Dan, WANG Hong-xin, SU Yu-hong, YANG Yu-hong, WANG Hao. Inhibition effects of U50, 488H on hypertrophic myocardial cells induced by isoproterenol[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(5): 537-541.

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Inhibition effects of U50, 488H on hypertrophic myocardial cells induced by isoproterenol

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