Effects of tubeimoside isolated from Bolbostemma paniculatum on microtubule in CNE-2Z cells

Abstract

Abstract: AIM: To examine the effects of tubeimoside isolated from Bolbostemma paniculatum on microtubule in low differentiated nasopharyngeal carcinoma CNE-2Z cells. METHODS: The interactions of tubeimoside with microtubule in CNE-2Z cells were investigated by immunofluorescence microscopy, and the interactions of tubeimoside with tubulin in CNE-2Z cells were detected by Western blotting. RESULTS: Tubeimoside (25 μmol·L^-1, 3 h) was sufficient to cause the microtubular network disruption. Western blot analysis revealed that the proportion of cytosolic tubulin of cells treated with tubeimoside increased in a time-dependent and concentration-dependent manner. CONCLUSION: Tubeimoside is an effective antimicrotubule agent.

Key words: Bolbostemma paniculatum, tubeimoside, microtubule, tubulin, antimicrotubule agent, CNE-2Z cells

CLC Number:

R979.1

SONG Gang, MA Run-di, YU Li-jian. Effects of tubeimoside isolated from Bolbostemma paniculatum on microtubule in CNE-2Z cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(6): 617-621.

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