Abstract: AIM :To study the role of NF-kappa B in regulating the expression of adhesion molecule ICAM-1 . METHODS:HUVECs were isolated from human umbili- cal veins by 0 .25 % trypsin and then cultured in RP- MI1640, exposed on LPS ( 100 ng·ml ^-1 ) for different time ( 1, 4, 8, and 24 h) , while pretreated by NF-kappa 中国临床药理学与治疗学 2006 Jan;11( 1) · 57 · B inhibitor pyrrolidine dithiocarbamate ( PDTC) at the 4th hour.Nucleus protein and total RNA were extracted .The activity of NF-kappa B and the expression of ICAM-1 were measured respectively by the electrophoretic mobility shift assay ( EMSA ) and reverse transcriptase-PCR ( RT- PCR) .RESULTS :The activity of NF-kappaB altered in correlate with ICAM-1 .Treating HUVECs with PDTC, the expression of ICAM-1mRNA was significantly down- regulated with the decline activity of NF-kappa B ( 2 .40 ±0 .11 vs 0 .76 ±0 .09, P <0 .01) .CONCLUSION: NF-kappa B plays an important role in regulating the ex- pression of ICAM-1Mrna .

Key words: Lipopolysaccride , ICAM-1 , NF-kappa B, PDTC