AQP4 deficiency alters regulative effects of estrogen on monoamine neurotransmitters

Abstract

Abstract: AIM: To investigate whether aquaporin-4(AQP4)participates in the regulative action of estrogen on neurotransmitters (NT).METHODS: The monoamine neurotransmitters were determined by high performance liquid chromatography (HPLC)in different brain regions of male AQP4-knockout CD1 mice and wild-type CD1 mice after they were intraperitoneally injected with different doses of estradiol.RESULTS: The contents of DA and 5-HT in the striatum and NE in the cortex of AQP4-knockout CD1 were significantly increased , and the concentrations of NE in the hippocampus and DA in the hypothalamus were decreased.Treatment with estradiol increased the contents of DA and 5-HT in the striatum and that of 5-HT in the hippocampus , decreased the levels of DA and 5-HT in the cerebrum, and also reduced the concentrations of DA and NE in the hypothalamus in wildtype mice.However , these changes were not found in AQP4-knockout mice.CONCLUSION: The deficiency of AQP4 in mice alters the regulative effects of estrogen on monoamine neurotransmitters , indicating that AQP4 participates in regulating metabolism of neurotransmitters.

Key words: aquaporin 4, knockout, monoamine neurotransmitter, estrogen

CLC Number:

R966

LI Ping, ZHANG Jing, GAO Lin, FAN Yi, SUN Xiu-lan, DING Jian-hua, HU Gang. AQP4 deficiency alters regulative effects of estrogen on monoamine neurotransmitters[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(11): 1256-1260.

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