Welcome to Chinese Journal of Clinical Pharmacology and Therapeutics,Today is Chinese

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2006, Vol. 11 ›› Issue (3): 292-295.

Previous Articles     Next Articles

Study on metabolites of scutellarin in gastrointestinal tract by UPLC-MS/MS method

JU Wen-zheng1,2, CHU Ji-hong1, TAN Ren-xiang2, XIONG Ning-ning1   

  1. 1Department of Clinic Pharmacology,Affiliated Hospital,NanjingUniversity of Traditional Chinese Medicine; United Laboratory in Jiangsu Province and Anhui Province for the Clinical Pharmacokinetics of Traditional Chinese Medicine and Westen Medicine,Nanjing 210029,Jiangsu,China;
    2School of Life Science,Nanjing University,Nanjing 210093,Jiangsu,China
  • Received:2006-01-05 Revised:2006-01-23 Online:2006-03-26 Published:2020-12-04

PDF (PC)

134

Abstract: AIM: The metabolites of scutellarin were studied in gastrointestinal tract.METHODS: Scutellarin was hydrolyzed with hydrochloric acid andβ-glucuronidase.Human intestinal flora and scutellarin were incubated in vitro.The metabolites were identified by UPLC-MS/MS method.RESULTS: Scutellarin could be hydrolyzed with hydrochloric acid andβ-glucuronidase,and could be metabolized by human intestinal bacteria.A main metabolite,scutellarein,also was found in the plasma of healthy volunteers after oral administration.CONCLUSION: There were scutellarin and scutellarein in intestinal before absorption.It was suitable for scutellarin pharmacokinetic studies to determine scutellarein or total aglycone.

Key words: scutellarin, UPLC-MS/MS, acid hydrolysis, β-glucuronidase, intestinal bacteria metabolism

CLC Number: