Effects of insulin treatment on content of intramyocellular triglyceride and expression of CPTI-βgene in skeletal muscle of diabetic mellitus rats

Abstract

Abstract: AIM: To study the effect of insulin administration on the content of triglyceride and the expression of carnitine palmitoyl transferase I-β(CPTI-β) gene in skeletal muscle of diabetic mellitus (DM) rats. METHEDS: Wistar rats were randomly divided into normal control group(NC, n =10) , diabetes with high fat diet (DH, n =10) , and diabetes with high fat diet plus insulin therapy (DHI, n =10).The fasting plasma glucose(FPG) , insulin (FPI) , free fatty acids (FFAs) ,triglyceride (TG) and the TGm (intramyocellular triglyceride) and the expression of CPTI-βgene in skeletal muscle were measured.HOMA2-IR was computed on the basis of FPI and FPG.RESULTES: Compared with NC group, HOMA2-IR, TG and FFAs levels in the plasma and TGm contents of DH groupwere significantly higher (p<0.01) , and the expression of CPTI-βgene was decreased (p<0.01).Compared with DH group, insulin administration didn't normalize TG (p>0.05) , but FFAs were decreased (p<0.01) , and TGm were increased (p<0.01) , although it improved insulin sensitivity of DHI group(p<0.05) , and the expression of CPTI-βwere increased (p<0.01).CONCLUSION: TG is accumulated in the skeletal muscle of DM rats, and the expression of CPTI-βis decreased, too.Insulin administration is able to up-regulate CPTI-βtranscription, although it can increase the content of TGm as well.This phenomenon suggests insulin can ameliorate triglyceride metabolize in the DM rats.

Key words: diabetes mellitus, insulin, triglyceride, carnitine palmitoyl transferase I-β, skeletal muscle

CLC Number:

WU Qian, CHEN Lu-lu, WANG Bao-ping, WEI Cui-feng, ZHOU Min, LI Yan. Effects of insulin treatment on content of intramyocellular triglyceride and expression of CPTI-βgene in skeletal muscle of diabetic mellitus rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(9): 1065-1068.

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