Hepatotoxicity and its mechanism of saikosaponin d on the human liver LO2cells in vitro

Abstract

Abstract: AIM: This study was to determine whether saikosaponin d (ssd)caused hepatotoxicity and to investigate the possible toxicity mechanism. METHODS: Experiment was carried out using the human live cell lines (L-O2)in vitro.MTT assay, change of cellular morphology, DNA fragmentation analysis, nuclear staining with 4, 6-diamidino-2-phenylindole (DAPI), hemolysis assay and membrane leakage of lactate dehydrogenase (LDH assay) were adopted to evaluate the hepatotoxicity of ssd. RESULTS: Results showed that L-O2 cells were inhibited significantly by above 2.5 μmol/L ssd, and IC50 = 2.44 μmol L.The change of celluar morphology, increase of LDH release and hemolytic ratio (%)could be detected significantly when treated with 5 μmol/L ssd. However, other results showed no obvious induction on the apoptosis from DNA ladder and DAPI. CONCLUSION: The toxicity mechanism of ssd on the L-O2 cells in vitro might be not through apoptosis, but related to the cytolysis.

Key words: saikosaponin d, huamn liver cell L-O2, hepatotoxicity in vitro

CLC Number:

R965.2
R966

LI Tao, JIANG Zhen-zhou, WANG Tao, ZHANg/Lu-yong, XU Xiao-yue, JIA Xiao-ming, MIAO Wen-ying, MIN Chao. Hepatotoxicity and its mechanism of saikosaponin d on the human liver LO2cells in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(4): 396-400.

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