Abstract: AIM: To explore mechanism of apoptosis in Eca-109 cells induced by 2-(3-carboxy-1-oxoprogy1)amino-2-deoxy-D-glucose (COPADG ). METHODS: Eca-109 cells were cultured with different concentrations of COPADG for 24 h. Then we examined cell growth inhibitory rate , apoptosis rate , reactive oxygen species(ROS)and ΔΧm of Eca-109 cells. RESULTS: The apoptosis rate of Eca-109 cells and the concentration of COPADG presented a positive correlation (rs=1.0 , P<0.01). The ΔΧm and the apoptosis rate of Eca-109 cells were related (rs=1.0 , P<0.01). The ROS and the apoptosis rate showed a positive relationship (rs=1.0 ,P<0.01). The levels of ROS and the ΔΧm were negatively related (rs=1.0 , P<0.01). CONCLUSION: COPADG promotes apoptosis of Eca-109 cells , raises ROS level in Eca-109 cells and lows level of ΔΧm. The experimental results suggest COPADG increases ROS level and lows level of ΔΧm triggering the thoroughfare of cell apoptosis to induce apoptosis in Eca-109 cells. The exaltation of ROS results in a decrease in ΔΧm.

Key words: 2-(3-carboxy-1-oxoprogy1)amino-2-deoxy-D-glucose, human esophageal cancer cell line Eca-109, apoptosis, reactive oxygen species, mitochondrial membrane potential