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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2007, Vol. 12 ›› Issue (5): 548-551.

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Effects of cinobufacine on intracellular adriamycin accumulation in human breast cancer cells

WANG-Ling1, LIU Shi-kun2, ZHOU Yu-lu2, PEI Qi2, YANG Ning3   

  1. 1Yongzhou Vocation-technical college, Yongzhou 425006, Hunan, China;
    2Department of Pharmacy, the Third Affiliated Hospital of Xiangya, Zhongnan University, Changsha 410006, Hunan, China;
    3Yongzhou People's Hospital, Yongzhou 425006, Hunan, China
  • Received:2006-12-30 Revised:2007-03-30 Published:2020-10-29

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Abstract: AIM: To study the effects of cinobufacine on intracellular adriamycin accumulation in human breast cancer MCF-7/ADM cells, and further explore the mechanism of cinobufacine reversing the multidrug resistance of human breast cancer MCF-7/ADM cells. METHODS: The cytotoxic effect was determined by MTT assay. Intracellular ADM concentration was examined by HPLC. RESULTS: 15 mg/L cinobufacine decreased the IC50 of ADM to MCF-7/ADM cells from 38.14 mg/L to 12.93 mg L, but increased intracellular adriamycin accumulation in MCF-7/ADM cells. CONCLUSION: Cinobufacine can increase intracellular ADM accumulation and partly reverse MDR of MCF-7/ADM cell line.

Key words: cinobufacine, adriamycin, multidrug resistance, MCF-7/ADM cell

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