Effects of HECCM and DDPH on the expression of osteopontin gene and α-SM-actin in PASMC

Abstract

Abstract: AIM: To study the effects of hypoxial endothelia cell conditional medium(HECCM) and DDPH on the expression of osteopontin(OPN) gene and α-SM-actin in pulmonary arterial muscle cell (PASMC), and the reverse effect of DDPH on proliferating PASMC induced by HECCM.METHODS: Hypoxial endothelia cell model was established in PASMC of swine.MTT assay and Immunocytochemistry was used to test cell proliferation, the expression of α-SM-actin.Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of OPN gene.RESULTS: (1) HECCM decrease the level of α-SM-actin and up-regulate the expression of OPN gene in PASMC;(2) DDPH increase the level of α-SM-actin and down-regulate the expression of OPN gene in PASMC.CONCLUSION: DDPH could reverse the phenotype transformation of PASMC exposed to HECCM, and transform PASMC from synthetic phenotype to normal contractile phenotype.

Key words: DDPH, α-smooth muscle-actin, hypoxia, osteopontin, pulmonary arterial smooth muscle cell

CLC Number:

R965.2

DU Bing, CHEN Pei-pei, WANG Xi-ming, DUAN Qiu-hong, HE Shan-shu. Effects of HECCM and DDPH on the expression of osteopontin gene and α-SM-actin in PASMC[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(9): 1013-1017.

References

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