Effects of Shenmai injection on the expression of Fas/FasL mRNA in pulmonary injury induced by ischemia-reperfusion in rabbits

Abstract

Abstract: AIM: To investigate the effects of Shenmai injection (SM) on the expression of Fas/FasL mRNA during pulmonary ischemia and reperfusion injury (PIRI) in rabbits.METHODS: Lung ischemia and reperfusion animal orthotopic model was established in vivo.The rabbits were randomly divided into three groups:sham operated group (sham, n=10), ischemia-reperfusion group (I-R, n=10) and I-R +Shenmai injection group (SM, n=10).The changes in several parameters, which included Fas/FasL mRNA locating expression, apoptotic index (AI), wet to dry ratio of lung tissueweight(W D) and index of quantitative assessment of histologic lung injury(IQA), and the histomorphology under light and electron microscope, were evaluated at 3 h after reperfusion in lung tissue.RESULTS: Compared with I-R group, the Fas/FasL mRNA slightly expressed in intima and extima of small pulmonary artery, pulmonary vein, and alveolar epithelium, bronchiole epithelia in SM group.The values of AI, W D and IQA were significantly lower than those in I-R group at 3 h after reperfusion in lung tissue (P <0.01 and P <0.05), and the changes of lung tissue in histomorphology were less markedly in SM group.CONCLUSION: Shenmai injection has notable protective effects on PIRI in rabbits, which may related with inhibition on Fas/FasL mRNA expression in lung tissue and decreasing apoptosis.

Key words: reperfusion injury, lung, Fas/FasL, apoptosis, shenmai injection

CLC Number:

R965.2

WANG Jun, LIN Li-na, WANG Wan-tie, JIN Li-da, QIU Xiao-xiao, DAI Yong-yue, WANG Qin. Effects of Shenmai injection on the expression of Fas/FasL mRNA in pulmonary injury induced by ischemia-reperfusion in rabbits[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(12): 1355-1359.

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