Abstract: AIM: To investigate the effects of Fas ligand (FasL) gene transduction mediated by adenoviral vector on the density of medial layer vascular smooth muscle cell after rat carotid artery injury.METHODS: SD rats were divided into treatment group (Ad-FasL group) and normal control group(Ad-βgal group) (n=6).Ad-βgal, Ad-FasL gene were transduced into endomembrane of balloon injury of rat carotid artery using recombination adenovirus vector, the density of medial layer vascular smooth muscle cell was observed after 3, 14 days, respectively.RESULTS: Compared with the normal control group, the density of medial layer vascular smooth muscle cell decreased after injury for 3 days, and returned to normal levels after injury for 14 days.CONCLUSION: FasL gene transduction into the vessel wall could decrease the density of medial layer vascular smooth muscle cell and inhibit the endomembrane accrementition.

Key words: Fas ligand, vascular smooth muscle medial cell density, adenovirus vector, gene therapy