Mistletoe induces apoptosis by down-regulating the constitutive activation of nuclear factor-κB in HCT116 cells

Abstract

Abstract: AIM: To investigate the role of NF-κB in apoptosis induced by mistletoe in human colon cancer HCT116 cells.METHODS: The cell growth inhibiting was measured by MTT assay, the cell apoptosis was observed by the agarose gel electrophoresis.The protein expression was detected by Western Blotting. DIG-EMSA was conducted to detemine the DNA-binding activation of NF-κB.RESULTS: Mistletoe had a remarkable growth inhibitory action on HCT116 cells. After exposed to mistletoe, HCT116 cells presented some morphologic features of apoptosis including cell shrinkage, nuclear condensation and DNA fragmentation.NF-κB was inhibited in cancer cells treated with mistletoe.Mistletoe may potentiate the cytotoxic effects of chemotherapeutic agents such as HCPT.CONCLUSION: Mistletoe may induce apoptosis in HCT116 cell line.Inhibitory action of NF-κB may play a significant role in apoptosis induced by mistletoe.

Key words: mistletoe, HCT116 cell line, NF-κB, apoptosis

CLC Number:

R363

LIN Lei, YE Meng, WANG Shao-ming, NI Shu-min, WU Xiao. Mistletoe induces apoptosis by down-regulating the constitutive activation of nuclear factor-κB in HCT116 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(7): 730-734.

References

[1] Urech K, Buessing A, Thalmann G, et al. Antiproliferative effects of mistletoe (Viscum album L. ) extract in urinary bladder carcinoma cell lines[J]. Anticancer Res, 2006, 26(4b): 3049-3055.
[2] 王少敏, 叶孟, 倪曙民. 槲寄生凝集素对人结肠癌HT-29 细胞增殖及凋亡的影响[J]. 中国临床药理学与治疗学, 2007, 12(9): 1028-1031.
[3] Kim WH, Park WB, Gao B, et al. Critical role of reactive oxygen species and mitochondrial membrane potential in Korean mistletoe lectin-induced apoptosis in human hepatocarcinoma cells[J]. Mol Pharmacol, 2004, 66(6): 1383-1396.
[4] KarinM. Nuclear factor-kappaB in cancer development and progression[J]. Nature, 2006, 441(7092): 431-436.
[5] Dutta J, Fan Y, Gupta N, et al. Current insights into the regulation of programmed cell death by NF-kappaB[J]. Oncogene, 2006, 25(51): 6800-6816.
[6] Nakanishi C, Toi M. Nuclear factor-kappaB inhibitors as sensitizers to anticancer drugs [J]. Nat Rev Cancer, 2005, 5(4): 297-309.
[7] Pacifico F, Leonardi A. NF-kappaB in solid tumors[J]. Biochem Pharmacol, 2006, 72(9): 1142-1152.
[8] Gupta S, Afaq F, Mukhtar H. Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cy cle arrest and apoptosis by apigenin in human prostate carcinoma cells[J]. Oncogene, 2002, 21(23): 3727-3738.
[9] 叶孟, 林蕾, 王少敏, 等. 槲寄生对人大肠癌HCT116细胞细胞周期作用的研究[J]. 现代实用医学,2007, 19(10): 780-784.
[10] 叶孟, 林蕾, 方勇, 等. 羟基喜树碱依赖Caspase3 途径诱导人乳腺癌Bcap37 细胞凋亡[J]. 中国临床药理学与治疗学, 2005, 10(7): 881-884.
[11] Freudlsperger C, Thies A, Pfuller U, et al. The proteasome inhibitor bortezomib augments anti-proliferative effects of mistletoe lectin-I and the PPAR-gamma agonist rosiglitazone in human melanoma cells[J]. Anticancer Res, 2007, 27(1A): 207-213.
[12] TanakaA,Muto S, Konno M, et al. A new IkappaB kinase beta inhibitor prevents human breast cancer progression through negative regulation of cell cycle transition[J]. Cancer Res, 2006, 66(1): 419-426.

[1]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[2]	YANG Xiaojia, JIANG Meng, WU Min, ZHANG Yili, LV Lan, WU Yuanfen, WANG Xinyu, LIU Liquan. Improvement effect of crocin on cognitive impairment of Alzheimer's disease rats through DKK3 regulation of GSK-3 β/β-Catenin pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 489-497.
[3]	HOU Ruiying, GUO Lige, JIAO Weijie, DU Lei, WU Guiyue, ZHAO Xu. Effects of Xiaokeshu recipe on levels of serum inflammatory factors in type2 diabetic rats and its mechanism [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(4): 377-382.
[4]	ZHANG Xuejiao, LIU Jieting, LI Luxin, CHEN Peijian, DING Minglu, SUN Mengwei, CHU Yanhui, ZHANG Zhen . Effects and mechanism of dapagliflozin on myocardial injury in type 1 diabetes mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 257-265.
[5]	HOU Xiaoyu, LENG Yufang, CAO Xuefen, LV Xingjiao, HAN Xiaoxia, Janvier NIBARUTA, LIU Yongqiang. Network analysis and experimental verification of Schisandrin B reduces intestinal ischemia reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 147-154.
[6]	WANG Jian, SUN Yongdong, ZHOU Xingwei, LIU Lei, CHEN Long, TONG Xingke, ZHU Jiali. Baicalein promotes laryngeal cancer cell death and inhibits invasion via miR-125b-5p/IRF4 axis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1209-1218.
[7]	ZHU Ruifang, GUO Dongkai, ZHI Hui, JIANG Yiguo, ZHANG Yueling, QIAN Xiaoping, JI Shiliang. Exploring the mechanism of elemene synergistic bortezomib against multiple myeloma by ROS-NF-κB-p38MAPK pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1219-1226.
[8]	ZHOU Huan, XU Ming, LI Bo, LIU Chunying, WANG Chun. PI3K/Akt/FOXO3a signaling pathway inducing protective autophagy promotes acquired lung adenocarcinoma resistance remodeling to DDP [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(9): 961-970.
[9]	HUA Haiyan, YAN Jie, QIN Yufen. Experimental study of schisandrin B attenuates acute myocardial ischemia injury in rats by inhibiting cardiomyocyte apoptosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 848-856.
[10]	JIN Yiyi, ZHOU Keting, YANG Chengcheng, XU Ping, ZHU Suyan. Osthole attenuates diabetes-induced renal injury by regulating NF-κB and p38/MAPK pathway mediated inflammatory responses [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 622-631.
[11]	LI Xueheng, LI Long. Research progress on the relationship between melatonin and idiopathic pulmonary fibrosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 715-720.
[12]	YANG Liu, LUO Ailin, LI Shiyong. Advance of general anesthetics-induced developing brain injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(12): 1381-1390.
[13]	TAN Fenglang, REN Hongli, WANG Hongfei. Effect of Phellodendri amurense extract on hippocampal nerve cells induced by Aβ_1-42 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1213-1220.
[14]	PAN Rongrong, ZHI Yinghao, JIN Yongxi, ZHOU Xiahui. Influences of theaflavins on neuronal apoptosis and blood-brain barrier in rats with cerebral hemorrhage by regulating CaMKK2/AMPK signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1240-1246.
[15]	LIU Peng, WANG Chen, WANG Yun, QIU Xinping. Dioscin attenuates inflammatory injury in uric acid-induced renal tubular epithelial cells by suppression of NF-κB signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(10): 1099-1105.