Study on the signal pathway of cell apoptosis in HT-29 cells and the gene expression of AQP3 induced by sodium butyrate

Abstract

Abstract: AIM:To investigate the signal pathway of cell apoptosis inHT-29 cells and the gene expression of AQP3 induced by sodium butyrate.METHODS: MTT method was used to detect the inhibitive effect of cell growth induced by different concentrations of sodium butyrate, while RT-PCR to detect the gene expressions of Cyclin D1, CDK4, p16 and AQP3, FCM for cell cycle detection.RESULTS:With the concentration of sodium butyrate more than 2.5 mmol/L, the cell growth of HT-29 cells was inhibited, the expressions of Cyclin D1, CDK4 and AQP3 were down-regulated while the expression of p16was up-regulated, cells were arrested in G₁/S.CONCLUSION:The apoptosis of HT-29 cells may be induced by sodium butyrate with concentrations more than 2.5 mmol/L, while the cell cycle arrested in G₁/S dose-dependently.The signal pathway of cell apoptosis may be the p16-Cyclin D1/CDK4 way, together with the down-regulation of AQP3.

Key words: sodium butyrate, HT-29, cell apoptosis, G₁/S arrested, signal pathway, AQP3

CLC Number:

R965.2

ZHU Jing, HONG Hua-rong, LIU Xiao-qun, ZHOU Xiao-li, CHEN Jian-li. Study on the signal pathway of cell apoptosis in HT-29 cells and the gene expression of AQP3 induced by sodium butyrate[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(8): 895-899.

References 10

[1]	Pajak B, OrzechowskIA, Gajkowska B.Molecular basis of sodium butyrate-dependent proapoptotic activity in cancer cells[J].Adv Med Sci, 2007, 52(1):83-88.
[2]	Fu D, LuM.The structural basis of water permeation and proton exclusion in aquaporins[J].Mol Membr Biol, 2007, 24(56):366-374.
[3]	Schrier RW.Aquaporin-related disorders of water homeostasis[J].Drug News Perspect, 2007, 20(7):447-· 898· Chin J Clin Pharmacol Ther 2008 Aug;13(8)453.
[4]	关兵.水通道蛋白与肿瘤[J].中国肿瘤临床, 2005,32(13):777-780.
[5]	Malumbres M.Cy clins and related kinases in cancer cells [J].J BUON, 2007, 12(1):S45-52.
[6]	Maddika S, Ande SR, PanigrahiS, et al.Cell survival, cell death and cell cycle pathways are interconnected :implications for cancer therapy [J].Drug Resist Updat, 2007, 10(12):13-29.
[7]	Berthet C, Kaldis P.Cell-specific responses to loss of cyclin-dependent kinases[J].Oncogene, 2007, 26(31): 4469-4477.
[8]	Patel RS, Rose B, Bawdon H, et al.Cyclin D1 and p16expression in pleomorphic adenoma and carcinoma ex pleomorphic adenoma of the parotid gland[J].histopathology, 2007, 51(5):691-696.
[9]	Wu B, Beitz E.Aquaporins with selectivity for unconventional permeants[J].Cell mol/L ife Sci, 2007, 64(18): 2413-2421.
[10]	秦佳敏.消化系统水通道蛋白[J].第三军医大学学报, 2005, 27(15):1612-1614.

[1]	ZHANG Xuejiao, LIU Jieting, LI Luxin, CHEN Peijian, DING Minglu, SUN Mengwei, CHU Yanhui, ZHANG Zhen . Effects and mechanism of dapagliflozin on myocardial injury in type 1 diabetes mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 257-265.
[2]	LI Fei, DING Huiqin, CHEN Mengjing. Isonlosinine induces autophagy of PC9 cells by ERK signaling pathway in lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1235-1240.
[3]	HUANG Huizhen, HAN Lei, LIN Xiaodong, CHEN Lei. Research progress on signal pathways related to the pathogenesis of pancreatitis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(1): 109-113.
[4]	CHEN Linjun, CHEN Wenbin. Jolkinolide B inhibits the proliferation and metastasis of colon cancer cells via blocking PI3K/Akt/NF-κB pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(3): 250-257.
[5]	ZHANG Danyang, DONG Xiaomin, ZHOU Xiaohuan, ZHANG Yue, ZHANG Juan, ZHOU Hebin. Gene microarray Analysis of Glucocorticoid resistance-related genes in Acute Lymphoblastic Leukemia based on GEO [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(3): 272-277.
[6]	SUN Hongya, YUAN Aijuan, LI Jipeng. Inhibitory effect of juglone on proliferation and invasion of human ovarian cancer SKOV3 and IOSE80 cells and its mechanism [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(1): 32-37.
[7]	. Effects and mechanisms of nicotinic acid curcumin on apoptosis induced by low shear stress in human umbilical vein endothelial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(8): 855-861.
[8]	WANG Yang, DU Pei, GAO Keqin, YANG Shuang, JIA Sujie. Effects of trichostatin A on CD14+ monocytes activation and TLR4 signal pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(8): 867-873.
[9]	HAN Chao, YANG Liu, ZHANG Qiuling, PAN Jinqiang, XU Jun. Effect of baicalin on airway remodeling of bronchial asthma [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(7): 749-754.
[10]	MA Chenghua, ZHOU Haijing, SHI Wen, WANG Zhiqiang, YANG Lan, CHEN Ping, ZHENG Xueli, MA Rui, MA Lijuan. Mechanism of apoptosis of mucoepidermoid carcinoma MEC-1 cells induced by ethanol extract of Lamiophlomis rotate [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(3): 253-259.
[11]	LUO Ziyin, XIANG Bingqian, GAO Hui, QIU Xiaoxiao, QIAN Xiaoying, WANG Wantie. Effects of dexmedetomidine on JNK in A549 cells with hypoxia/reoxygenation injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(12): 1346-1351.
[12]	SHAO Mei-qin, CHEN Hai-e, HE Jin-bo, MA Ying-chun, HUANG Lin-jing, CHEN Dan, WANG Yang, WANG Wan-tie. Effects of JNK on ischemic postconditioning attenuating pneumocyte apoptosis after lung ischemia/reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(5): 531-536.
[13]	LI Zhao-fa, WANG Cong-yang, DENG Xiao-ying, HUI Er-jing. Infusion receptor eDR4/iFas enhances TRAIL-induced apoptosis of HT-29 cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 966-972.
[14]	ZHOU Jie, YAN Ling, HUANG Duo-xin. Mfn2 gene transfer inhibites neonatal rat cardiomyocytes apoptosis induced by ischemia/reperfusion via activation of the Ras-PI3K-Akt signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 973-978.
[15]	CHENG Zhi-yun, PENG Jun-chun, LV Ying-hui. Role of epithelial-mesenchymal transition in the liver fibrosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(8): 928-936.