Effect of ischemia injury on microcirculation of bile ducts relating to liver transplantation and protective effect of recombinant human growth hormone

Abstract

Abstract: AIM: To investigate the changes of microcirculation of bile ducts relating to liver transplantation in rats with ischemia injury and protective effect of recombinant human growth hormone (rhGH) on ischemia injury. METHODS: The rat orthotopic autologous liver transplantation was performed firstly. Wistar rats were randomly divided into three groups each with 6 rats including control group (CON), ischemia injury group (HLA) and rhGH group.Specimen of liver tissue was collected after 7 days post-operation. The blood vessels were tagged with rabbit anti-CD34 polyclonal antibody.The blood vessels and bile ducts of portal area were observed and enumerated. RESULTS: In HAL group, the array of intrahepatic cholangiocytes was significantly absent.There was no obvious soakage of inflammatory cells in portal area. The number of blood vessels and bile ducts in portal area was significantly decreased.The number of bile ducts not accompanying with blood vessels was significantly increased (P <0.05);In rhGH group, the changes of chronic hyperplastic inflammation were oberserved in intrahepatic cholangiocytes.The number of blood vessels and bile ducts in portal area was significantly increased.The number of bile ducts accompanying with blood vessels was significantly increased (P <0.05). CONCLUSION: Ischemia injury of bile ducts relating to rat orthotopic autologous liver transplantation damnifies the microcirculation of intrahepatic bile ducts.However, administration of exogenous rhGH promotes recovery of intrahepatic microcirculation consequently protecting bile ducts from ischemia injury.

Key words: liver transplantation, bile ducts, microcirculation, recombinant human growth hormone

CLC Number:

R619.5

WANG Zheng, ZHOU Jie, LIN Yi-xiong, CUI Zhong-lin, ZHANG Qi-fan. Effect of ischemia injury on microcirculation of bile ducts relating to liver transplantation and protective effect of recombinant human growth hormone[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1221-1224.

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