Protective effect of Cistanchis glycosides on ethanol-induced liver damage in mice

Abstract

Abstract: AIM: To research the protection of Cistanchis glycosides on ethanol-induced liver damage in mice. METHODS: 40 mice were divided into 4 groups in random, and administered by gavage for 4 weeks.Cistanchis glycosides were twice a day, ethanol were once a day.When time limit arrived, mice were weighted, the serum was afforded to detect the liver function index, liver homogenate were prepared for biochemical detections, liver remained were fixed for pathological investigation. RESULTS: When mice were damaged by ethanol, liver index, liver function index, contents of TG, TC, MDA and LPO were increased distinctly(P <0.05), The activities of SOD, GSH-Px and CAT were decreased at the same time(P <0.05).Imflammatory infiltration and flat vacuoles were observed.Cistanchis glycosides ameliorated these changes evidently(P <0.05);the effects were dosedependence. CONCLUSION: Protective effect of Cistanchis glycosides on ethanol-induced liver damage in mice is associated with antioxidation, enhancement of clearance for free radicals and metabolites, and inhibition of lipid overoxidation reactions.

Key words: Cistanchis glycosides, ethanol-induced liver damage, liver function, lipid overoxidation reactions

CLC Number:

R965.2

LUO Hui-ying, LIU Yuan, XI Guo-zhu, DU Jian-de. Protective effect of Cistanchis glycosides on ethanol-induced liver damage in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1225-1228.

References

[1] Quanbo X, Shigetoshi K, Tadato T, et al.Antioxidative effects of phenylethanoids from Cistanche deserticola[J]. Biol Pharm Bull, 1996, 19(12):1580-1585.
[2] Liwei L, Mingtsuen H, Fanhsiu T, et al.Anti-nociceptive and anti-inflammatory activity caused by Cistanche deserticola in rodents[J].J Ethnopharmacol, 2002, 83(3):177-182.
[3] Quanbo X, Yasuhiro T, Takuji K, et al.Inhibition of nitric oxide by phenylethanoids in activated macrophages[J].Eur J Pharmacol, 2000, 400(1):137-144.
[4] Adam M.Plant in vitro culture for the production of antioxidants[J].Biotech Advances, 2008, 26(6):548-560.
[5] Paula LH, Takeshi Y, Boris T, et al.Transgenic and gene “Knockout” models in alcohol research[J].Alcoholism:Clin Exp Res, 2006, 25(1):60-66.
[6] Yongke L, Arthur IC.CYP2E1 and oxidative liver injury by alcohol[J].Free Radical Bio Med, 2008, 44(5):723-738.
[7] James H, Servio HR, Nicholas F, et al.Mechanism of alcohol-induced oxidative stress and neuronal injury[J]. Free Radical Bio Med, 2008, 45(11):1542-1550.
[8] Thomas JW, Robert TC, Elizabeth JK, et al.Alcohol and inflammation and immune responses:summary of the 2006 Alcohol and Immunology Research Interest Group (AIRIG) meeting[J].Alcohol, 2008, 42(2):137-142.
[9] Yangming T, Yiru J, Ingjun C, et al.Roles of the genetic variation of alcohol-metabolizing enzymes on biomarkers in trauma patients with excessive alcohol intake at emergency department[J].Clin Chim Acta, 2008, 389(1):14-18.
[10] Sudheer KM, Adrienne LK, Kelly KA, et al.Mitochondrial dysfunction and oxidative stress in the pathogenesis of alcohol- and obesity-induced fatty liver diseases[J].Free Radical Bio Med, 2008, 44(7):1259-1272.
[11] Monica D, Alessandra I, Antonella R, et al.Protective effect of hydroxytyrosol and its metabolite homovanillic alcohol on H2O2 induced lipid peroxidation in renal tubular epithelial cells[J].Food Chem Toxicol, 2008, 46(9): 2984-2990.
[12] Jing Z, Hui C, Yan L.Protective effect of bicyclol on acute alcohol-induced liver injury in mice[J].Eur J Pharmacol, 2008, 586(1):322-331.
[13] Luisa V, Lorenzo L, Anna F, et al.Acute alcohol intoxication[J].Eur J Pharmacol, 2008, 19(8):561-567.
[14] Kavitha G, Damodara VR, Paramahamsa M, et al.Role of nitric oxide in alcohol-induced changes in lipid profile of moderate and heavy alcoholics[J].Alcohol, 2008, 42(1):47-53.
[15] 王福根, 俞佳, 席建军, 等.乙酰半胱氨酸对大鼠酒精性肝损伤的保护作用[J].中国临床药理学与治疗学, 2008, 13(5):517-520