Establishment and application of ligase-based method detecting polymorphism of TPMT

Abstract

Abstract: AIM: To establish one ligase-based method to detect the gene polymorphism of TPMT. METHODS: The multiplex PCR ligase detection reaction(LDR) was established, and 50 blood samples of healthy postulants were investigated with this method. RESULTS: This method was reliable, rapid, high-degree automatic.The genotype of ^＊2, ^＊3A, ^＊3B and ^＊4, which depressed the activity of TPMT were not found, only 2 samples of TPMT ^＊3C heterozygote were detected.CONCLUSION: This method can be applied for TPMT polymorphisms detection.

Key words: thiopurine S-methyltransferase, ligase detection reaction, azathiopurine, genetic polymorphism

CLC Number:

R965.2

LIANG Xin, ZHANG Ya-tong, CHENG Gang, HU Xin, SUN Ying-qi, YANG Chen, LI Kexin, SUN Chun-hua. Establishment and application of ligase-based method detecting polymorphism of TPMT[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1281-1285.

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