Study on the dose-effect relationship of Danggui-Shaoyao-San′s ingredients proportion in dysmenorrhea mice

Abstract

Abstract: AIM:To analyze the influence of different combinations and proportions of Danggui- Shaoyao-San′s ingredients in treating primary dysmenorrhea. METHODS:The dysmenorrhea mouse model was established by oxytocin. The ingredient compatibility was analyzed by orthogonal t design and the appropriate proportion relationship was researched by uniform design. The latent period and number of twisting body were recorded to evaluate the intensity of dysmenorrhea. The algomenorrhea effect was observed with the treatment of Danggui-Shaoyao-San or the combination of different ingredients in a prescription. RESULTS: The orthogonal t design experimental results showed the latent period was significantly prolonged by Paeonia lactiflora Pall. (B), Angelica sinensis Diels. (A)and Ligusticum chuanxiong Hort. (C), the number of twisting body was decreased by B. Poria cocos Wolf. (D), Alisma orientalis Juzep (E)and Atractylodes macrocephala Koidz. (F)had no significant influence on the latent period and the number of twisting body. The uniform design results showed the indexes were fitted with regression equation, the data of each variable had mild surface effect, the proper proportion between A∶B∶C was 8. 28∶13. 3∶4. 6 through surface analysis, and the above conclusion was proved. CONCLUSION: The influnce on twisting body in algomenorrhea mouse of B was obvious, while A, C take the second place, D E, F played auxiliary function. The study gave strong support to the original formula to put B in a very important position, and it was speculated that slightly modulating the proportion of ingredients might bring better effects. The orthogonal t design and uniform design are valuable methods in studying multi-ingredient formulae of traditional Chinese medicine.

Key words: Danggui-Shaoyao-San, dysmenorrhea, dose-effect relationship, orthogonal t design, uniform design

CLC Number:

R965. 2

HUA Yong-qing, XIE Hai-tang, DUAN Jin-ao, SU Shu-lan, QING Ping. Study on the dose-effect relationship of Danggui-Shaoyao-San′s ingredients proportion in dysmenorrhea mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(5): 557-563.

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