Effects of realgar bioleaching solution on the expression of MDRI mRNA and P-gp in K562/ADM cells

Abstract

Abstract: AIM: To investigate the role of apop-tosis in K562/ADM cells induced by realgar bioleaching solution (RBS), and illustrate the possible molecular mechanism .METHODS: RBS was prepared by a new method of bioleaching with bacteria .The cell apoptosis was determined by annexin V/PI double staining .The expressions of MDRI mRNA and P-gp were detected by RT-PCRand flow cytometry, respectively.The parallel experiments with arsenic acid (H₃AsO₃)were conducted for comparison .RESULTS: RBS inhibited K562/ADM cells growth effectively, and the apoptosis rate of the cells by Annexin V/PI staining was obviously in-creased, the expressions of MDRI mRNA and P-gp were significantly down-regulated. With the same con-centration of As, the inducing effect of RBS was more stronger than H₃AsO₃ .CONCLUSION: RBS induces the apoptosis inK562/ADM cells. The down-regulation of MDRI P-gp expression may be the important molecular mechanisms in reversing the multi-drug resistance leukemia cells.

Key words: realgar bioleaching solution, K562/ADM cell, apoptosis, P-gp, MDRI

CLC Number:

R965.2

ZHANG Jing-hong, LI Hong-yu. Effects of realgar bioleaching solution on the expression of MDRI mRNA and P-gp in K562/ADM cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(8): 855-860.

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