Effect of histamine on fat synthesis and regulation in the white adipocytes: a mechanism research

Abstract

Abstract: AIM: To study the effect of histamine on fat synthesis in the differentiated 3T3L1 cell in vitro and its mechanisms.METHODS: The 3T3L1 cells cultured and induced it to white fat cells.The levels of aP2 mRNA in the adipocytes under the treatment of histamine and diphenhydramine were detected by RT-PCR techniques.RESULTS: Histamine increased the expression of aP2 mRNA in the adipocytes, the concentration-dependent effect curve discovered the highest expression of aP2 mRNA was at 20 μmol/L, the time-dependent effect curve discovered the highest expression of aP2 mRNA was at 6 h.Compare with the same concentration of histamine, diphenhydramine decreased the expression of aP2 mRNA(P<0.05).CONCLUSION: Histamine increased the expression of aP2 mRNA in the differentiated 3T3L1 cell.Diphenhydramine inhibited the expression of aP2 mRNA. Histamine can increase the fat synthesis and maintain the balance of energy metabolism in the white adipocytes.

Key words: Histamine, Fat synthesis, aP2 mRNA

CLC Number:

R965.2

ZENG Hui, LIU Zhao-qian. Effect of histamine on fat synthesis and regulation in the white adipocytes: a mechanism research[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(12): 1343-1347.

References

[1] Attoub S,moizo L,sobhani I, et al. The H3 receptor is involved in cholecystokinin inhibition of food intake in rats [J]. Life Sci,2001,69(4):469-478.
[2] Lovenberg TW, Roland BL, Wilson SJ, et al. Cloning and functional expression of the human histamine H3 receptor[J]. Mol Pharmacol,1999, 55(6):1101-1107.
[3] Yoshimatsu H, Chiba S, Tajima D, et al. Histidine suppresses food intake through its conversion into neuronal histamine [J]. Exp Biol Med ,2002,227(1):63-68.
[4] Tsuda K, Yoshimatsu H, Niijima A,et al. Hypothalamic histamine neurons activate lypolysis in rat adipose tissue [J]. Exp Biol Med, 2002,227(3):208-213.
[5] Collins S, Cao W, Daniel KW,et al. Adrenoceptors, uncoupling proteins, and energy expenditure[J]. Exp Biol Med, 2001,226(11):982-990.
[6] Masaki T, Chiba S, Yoshimichi G, et al. Neuronal histamine regulates food intake, adiposity, and uncoupling protein expression in agouti yellow (ay/a) obese mice[J]. Endocrinology, 2003, 144(6):2741-2748.
[7] Avanzati B, Catala A. Partial purification of fatty-acid binding protein by ammonium sulphate fractionation[J]. Arch Int de Phtsiol Biochim , 1983, 91 (2):103-108.
[8] Becker MM,Kalinna BH,Waine GJ,et al. Gene cloning, overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein from the human blood fluke Schistosoma japonicum[J]. Gene,1994, 148 (2): 321-325.
[9] Kawazoe Y,Tanaka S,Uesugi M, et al. Chemical genetic identification of the histamine H1 receptor as a stimulator of insulin-induced adipogenesis [J]. Chem Biol , 2004, 11(7): 907-913.
[10] Deng C, Weston-Green K, Huang XF. The role of histaminergic H1 and H3 receptors in food intake: a mechanism for atypical antipsychotic-induced weight gain [J] ? Prog Neuropsychopharmacol Biol Psychiatry,2010,34(1):1-4.
[11] Choi S,Disilvio B,Unangst J,et al. Effect of chronic infusion of olanzapine and clozapine on food intake and body weight gain in male and female rats[J]. Life Sci, 2007,81 (12) :1024-1030.
[12] He RR, Yao N, Wang M, et al. Effects of histamine on lipid metabolic disorder in mice loaded with restraint stress[J]. J Pharmacol Sci, 2009 ,111(2):117-123.
[13] 刘昭前,杨敏. 过氧化物酶体增殖物活化受体δ及其在代谢综合征中的作用[J]. 中国临床药理学与治疗学, 2007,12 (11) :1205-1210.
[14] Bruce M King. The rise, fall, and resurrection of the ventromedial hypothalamus in the regulation of feeding behavior and body weight[J]. Physiol Behav, 2006,87(2):221-244.
[15] Machidori H, Sakata T, Yoshimatsu H, et al. Zuker obese rats: defect in brain histamine control of feeding [J]. Brain Res, 1992,590(1/2):180-186.
[16] Lovenberg TW, Roland BL, Wilson SJ, et al. Cloning and functional expression of the human histamine H3 receptor. [J] Mol Pharmacol, 1999,55(6):1101-1107.
[17] Wang KY, Tanimoto A, Yamada S,et al. Histamine regulation in glucose and lipid metabolism via histamine receptors: model for nonalcoholic steatohepatitis in mice[J]. Am J Pathol, 2010,177(2):713-723.
[18] Yoshinori K, Satoshi T, Motonari U, et al. Chemical genetic identification of the histamine H1 receptor as a stimulator of insulin-induced adipogenesis [J].Chem Biol, 2004,11(7):907-913 .
[19] Avanzati B, Catala A.Partial purification of fatty-acid binding protein by ammonium sulphate fractionation[J].Arch Int Phtsiol et de Biochim, 1983,91(2):103-108.