Abstract: AIM: To investigate the protective effects of 13-methyltetradecanoic acid(13-MTD) on the injury of cultured SH-SY5Y neural cells induced by hydrogen peroxide(H₂O₂). METHODS: The injury of cultured SH-SY5Y cells was made in vitro by H₂O₂. Microscope was used to detect the morphology of cells. Sulforhodamine B assay was applyed to detect the cell viability. Acridine orange/ethidium bromide (AO/EB) double staining was adopted to observe the apoptotic morphology. MTT assay was employed to detect the mitochondrial reducibility. RESULTS: The cells apoptosis rate was increased, the cells viability and mitochondrial reducibility were decreased significantly in group model of H₂O₂ injury compared with the group control (P<0.01). 13-MTD 10, 20, 40 μg/mL increased cell survival and 13-MTD 5, 10, 20 μg/mL reduced the apoptosis rate as well as 13-MTD 5, 10, 20, 40 μg/mL increased the cell MTT metabolic rate compared with the group model of H₂O₂ injury(P<0.01), there are some dose-dependent relationship (P<0.05, P<0.01). CONCLUSION: Different doses of 13-MTD shows the neuroprotection effects and can protect SH-SY5Y cells from the injury induced by H₂O₂.

Key words: 13-methyltetradecanoic acid, Hydrogen peroxide, SH-SY5Y, Cell viability, Mitochondrial reducibility, Apoptosis