Welcome to Chinese Journal of Clinical Pharmacology and Therapeutics,Today is Chinese

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2010, Vol. 15 ›› Issue (7): 732-736.

Previous Articles     Next Articles

Effect of tirofiban on the plasma inflammatory factors of P-selectin,IL-6 and IL-1β in vitro

XIE Shuang-feng1, YIN Song-mei1, NIE Da-nian1, LI Yi-qing1, XIAO Jie1, MA Li-ping1, WANG Xiu-ju1, WANG Jing-feng2   

  1. 1Department of Hematology, 2Department of Cardiology, Second Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510120, Guangdong, China
  • Received:2010-03-04 Revised:2010-06-24 Online:2010-07-26 Published:2020-09-15

Abstract: AIM: To explore the anti-inflammatory effects of tirofiban in vitro, and to provide the theoretical basis for properly using the GP Ⅱb/Ⅲa receptor antagonists.METHODS: The platelets in the blood samples were activated in vitro with adenosine diphosphate (ADP) or thrombin respectively. Tirofiban was added to each sample in 20%, 50% or 80% inhibiting concentrations. Then the mixed liquor was incubated in 37 ℃ for 30 minutes. After centrifugation, the samples were divided to supernatants and sediments. The P-selectin, interleukin-6 (IL-6) and interleukin-1β (IL-1β) of were detected by enzyme linked immunosorbent assay (ELISA).RESULTS: ADP (final concentration: 10 μmol/L) could induce the production of P-selectin, that could be inhibited by tirofiban in concentrations higher than 31.25 μmol/L (IC20). ADP had no effect on the production of IL-6 or IL-1β. Thrombin (final concentration: 0.03 U/L) could induce the production of P-selectin, IL-6 and IL-1β, those could be inhibited by tirofiban in concentrations higher than 156.25 μmol/L (IC50). Tirofiban without ADP or thrombin had no effect on the production of P-selectin, IL-6 or IL-1β.CONCLUSION: Tirofiban in concentration higher than IC50 could inhibit the production of P-selectin and inflammatory factors induced by thrombin. Tirofiban in full dosage could inhibit the activation of platelet and decrease the production of inflammatory factors at the same time in vitro.

Key words: Platelet, Tirofiban, Inflammatory factor

CLC Number: