HMGA2 play important roles both in tumorigenesis and angiogenesis

Abstract

Abstract: AIM: To analyze the roles of high mobility group A2 protein (HMGA2) in tumorigenesis and angiogenesis. METHODS: 5 siRNAs targeting HMGA2 gene (HMGA2 siRNA1-5) were designed, synthesized, and transfected into cells using Lipofactamine 2000. MTT assay, transwell assay and tube formation assay were applied to evaluate the effects of these siRNAs on cancer cells (SPC-A1 and Hela) and endothelial cells (ECV-304 and HUVEC). RESULTS: HMGA2 siRNA1, 3 and 5 inhibited the growth of all the cell lines that were tested. Among these siRNAs, HMGA2 siRNA5 show the best inhibition effect. The relative growth ratios were 63.2%±6.5% (P<0.01), 86.8%±3.5% (P<0.01), 58.5%±6.3% (P<0.01), and 60.3%±7.3% (P<0.01) for Hela, SPC-A1, EVC-304 and HUEVC cells, respectively. Furthermore, interfering the expression of HMGA2 affected the growth, migration, and tube formation of endothelial cells (P<0.01). CONCLUSION: HMGA2 protein plays important roles both in tumorigenesis and angiogenesis.

Key words: HMGA2, Angiogenesis, Tumorigenesis, RNAi, Gene therapy

CLC Number:

R965.2

LV Ying-hui, GONG Yu-hua, DIAO Yong, XU Rui-an, WANG Qi-zhao. HMGA2 play important roles both in tumorigenesis and angiogenesis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(9): 975-980.

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