Abstract: AIM: To investigate the effect of proanthocyanidins from grape seeds (GSPE) on c-Jun and c-Fos expression in colon tissues of recurrent colitis rats. METHODS: Wistar male rats were randomized divided into six groups: normal control group, model control group, Sulfasalazine (SASP) group, and the 3 GSPE treatment groups. Rats were intragastrically administered different doses of GSPE (100, 200, and 400 mg/kg) per day for 7 days after recurrent colitis was twice-induced by TNBS-50% ethanol. Rats were killed after GSPE treatment 7 days. The colonic injury and inflammation were assessed by macroscopic and microscopic damage scores and myeloperoxidase (MPO) activity. The expression levels of c-Jun and c-Fos in the colon tissues were measured by enzyme-linked immunosorbent assay methods. RESULTS: Compared with the model group, the macroscopic and microscopic damage scores and the activity of MPO were significantly decreased in GSPE dosage group. Moreover, the expression levels of c-Jun and c-Fos in the colon tissues were significantly decreased. CONCLUSION: GSPE can inhibit the expression of c-Jun and c-Fos in the colon tissues of the recurrent colitis rats.

Key words: Proanthocyanidins from grape seeds, 2,4,6-trinitrobenzenesulfonic acid, Colitis, Re- current, c-Jun, c-Fos