Abstract: AIM: To discuss the effect of photodynamic therapy(PDT)combined with anti-cancerdrugs cinobufotalin on transplanted H₂₂ liver cancer by observing its influence on motilin,gastrin. METHODS: The tumor inhibition rate and the levels of gastrin and motilin after Methyl esterification of chlorophyll (MECD)combined with cinobufotalin treating transplanted H₂₂ liver cancer were determined.RESULTS: The inhibitory rate of PDT combined with cinobufotalin Group,cinobufotalin Group, PDT Group was 51.69%,33.49%,26.50%,respectively.The motilin and gastrin were maintained at a certain level treated with PDT, it could reduce digestive tract reaction,such as nausea and vomit.CONCLUSION: Experiments show that PDT combined with cinobufotalin can strengthen obviously photosensitive killing effect on mice transplanted H₂₂ liver cancer, meanwhile,it can improve significantly the overall situation of mice. It has a practical significance and deserves recommendation.

Key words: Methyl esterification of chlorophyll (MECD), Photodynamic therapy(PDT), Cinobufotalin, Motilin, Gastrin