Evaluation on the function of MRP1 in pulmonary epithelial cells by the efflux level of phenolsulfonphthalein

Abstract

Abstract: AIM: To evaluate the function of MRP1 in pulmonary epithelial cells by the efflux level of substrate using phenolsulfonphthalein as MRP1 substrate and probenecid as MRP1-specific inhibitor. METHODS: The mouse was divided into the control group and inhibitor inhaled groups. The bronchoalveolar lavage fluid (BALF) and plasma samples were collected at 30 min after phenolsulfonphthalein (250 mg/kg) was administered to the control group mouse by intravenous injection. The inhibitor inhaled groups mouse was administered phenolsulfonphthalein (250 mg/kg) at 10, 30 and 60 min after inhaled probenecid (150 mg/kg), and the BALF and plasma samples were collected at 30 min after phenolsulfonphthalein administration. The phenolsulfonphthalein concentration in BALF and plasma was determined and the concentration of in BALF/plasma ratio was calculated.RESULTS: There was no difference in the plasma concentration of phenolsulfonphthalein between the control group and inhibitor groups. Compared with control group, the phenolsulfonphthalein concentration in BALF and the phenolsulfonphthalein concentration of in BALF/plasma ratio of the 60 min after inhaled probenecid group had significant difference.CONCLUSION: The efflux level of phenolsulfonphthalein in BALF could reflect the function of mouse pulmonary epithelial cells MRP1.

Key words: Phenolsulfonphthalein, Efflux level, MRP1, Probenecid

CLC Number:

R965.2

TAO Xiu-hua, WANG Dian-lei, CAO Yin, WANG Chen-yin, CHEN Jin-pei, YANG Li-li. Evaluation on the function of MRP1 in pulmonary epithelial cells by the efflux level of phenolsulfonphthalein[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(2): 127-131.

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