Effects of crocetin on ulcerative colitis in rats and the mechanism

Abstract

Abstract: AIM: To determine the effect of crocetin on ulcerative colitis(UC)induced by tri-nitrobenzene sulfonic acid(TNBS)in rats, and to explore the possible mechanism.METHODS: UC was induced by TNBS and ethanol in rats. After crocetin was given orally, the colon mucosa damage idex and histopathological score were determined, and the levels of MPO,MDA and SOD were measured. Tumor necrosis factor-α(TNF-α) and Interleukin-1β(IL-1β) in colon were detected by enzyme-linked immunosorbent assay(ELISA). Toll-like receptor4(TLR4) mRNA and nuclear factor-κBp65(NF-κBp65) mRNA expression were examined by Q-PCR. Immunohistochemistry staining was used to observe the expression of TLR4 and NF-κBp65.RESULTS: Crocetin greatly attenuated the severity of colonic gross lesions and reduced the histopathological scores. MPO activity and MDA concentration in colon were significantly increased,SOD activity was reduced in UC rats.Crocetin ameliorated these changes. Crocetin reduced the levels of TNF-α and IL-1β in colon. The expression of TLR4 and NF-κBp65 in colon were attenuated by crocetin.CONCLUSION: Crocetin can effectively attenuate the inflammatory reaction in UC rats,which may be due to the antioxidation and the inhibition of TLR4/NF-κB signal pathway.

Key words: Ulcerative colitis, Crocetin, TLR4, NF-κB

CLC Number:

R965.2

TAO Yun, GONG Guo-qing, QIAN Zhi-yu, CHENG Wen-yuan, WANG Xin-hui. Effects of crocetin on ulcerative colitis in rats and the mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(3): 263-270.

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