Apoptosis of RAW264.7 macrophages induced by chloral hydrate and its mechanisms

Abstract

Abstract: AIM: To investigate the effect of chloral hydrate treatment on the apoptosis of RAW264.7 macrophages and the mechanisms of apoptosis.METHODS: To treat RAW264.7 macrophages by chloral hydrate with different concentrations and different times, then to observe the morphological changes. Also, Annexin V-FITC/PI double staining assay, Hochest 33258 and and DNA ladder assay were used to detect the apoptosis, and the expression of Fas/FasL.RESULTS: After treatment with chloral hydrate, the morphology of RAW264.7 macrophages changed from fusiform to round, and suspension at last. Annexin V-FITC/PI double staining assay showed RAW264.7 macrophages were from early to late apoptosis after chloral hydrate treatment. Hochest 33258 and and DNA ladder assay showed chloral hydrate treatment can induced the apoptosis of RAW264.7 macrophages. Also, the apoptotic RAW264.7 macrophages expressed higher levels of Fas, but did not express FasL.CONCLUSION: Chloral hydrate treatment could induce RAW264.7 macrophages apoptosis via the Fas/FasL pathway.

Key words: chloral hydrate, RAW264.7 macrophages, apoptosis, Fas/FasL

CLC Number:

R965.2

PAN Qing-jun, ZHU Xue-zhi, LIU Yuan. Apoptosis of RAW264.7 macrophages induced by chloral hydrate and its mechanisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(2): 121-126.

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