Effects of salvia miltiorrhiza and electroacupuncture on the expressions of glial fibrilliary acidic protein and iNOS in rats with cerebral ischemia-reperfusion injury

Abstract

Abstract: AIM: To observe the effects of salvia miltiorrhiza(SM) and electroacupuncture (EA) on the expressions of glial fibrilliary acidic protein (GFAP) and iNOS in rats after focal cerebral ischemia-reperfusion.METHODS: 60 Sprague-Dawley rats were randomized into the sham opration, model, SM, EA, and SM+EA groups. The models rats received intraluminal middle cerebral artery occlusion(MCAO) with a nylon monofilament suture. SM injection at 5 g/kg were injected into abdominal cavity in the SM group, EA(2-15Hz, dilatational wave) at Baihui and Dazhui was performed to rats in the EA group, lasting for 30 min, SM+EA group was treated with both SM abdominal cavity injection and electroacupuncture therapy, once a day for 4 days.To observe the neurologic impairment score in each group, and the water content of brain tissue was determined by weighing. HE staining was performed to display the pathological changes of the brain tissues, and the expressions of GFAP and iNOS in striatum were detected by immunohistochemical method.RESULTS: The neurologic impairment score and water content of brain tissue in model group were significantly increased compared with sham operation group(P<0.01).Compared with model group, the neurologic impairment score and water content of brain tissue in SM group, EA group and SM+EA group were significantly decreased (P<0.05,P<0.01), and the score and water content in SM+EA group was obviously lower than those of the SM group and the EA group(P<0.05, P<0.01). The pathological changes of the brain tissues of four group rats were consistent with the above result. The expression of GFAP and iNOS in model group was obviously increased compared with sham operation group(P<0.01).Compared with model group, the expression of GFAP and iNOS in SM group, EA group and SM+EA group were significantly decreased (P<0.05, P<0.01). The expression of GFAP and iNOS in SM+EA group was obviously lower than those of the SM group and the EA group (P<0.05, P<0.01).CONCLUSION: SM and EA has a neuroprotective activity in cerebral ischemia-reperfusion neuronal injury, which is probably related to inhibit the excessive activation of astrocytes, decrease the expression of iNOS, besides, the effects of SM combined with EA was higher than the SM group or the EA group.

Key words: cerebral ischemia reperfusion, electroacupuncture, glial fibrilliary acidic protein, iNOS

CLC Number:

R965.2

ZHANG Ye-gui, ZHAO Jian, DING Yan-xia, HOU Liang-qin. Effects of salvia miltiorrhiza and electroacupuncture on the expressions of glial fibrilliary acidic protein and iNOS in rats with cerebral ischemia-reperfusion injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(2): 127-132.

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