Telithromycin suppresses nontypeable Haemophilus influenzae-induced MUC5AC expression via inhibition of activator protein-1 signaling

Abstract

Abstract: AIM: To investigate the effect of Telithromycin on nontypeable Haemophilus influenza (NTHi)-induced mucus MUC5AC expression in airway epithelial cells and underlying molecule mechanism.METHODS: Cultured NCI-H292 cells were pre-incubated with 10 mg/L of Telithromycin for 6 h, and then NTHi was added in different concentrations for 3-9 h. MUC5AC gene mRNA level and mucin protein expression were quantified by real time PCR and ELISA, respectively; DNA-binding activities of NF-κB and activator protein-1 (AP-1) were detected by ELISA.RESULTS: NTHi significantly increased MUC5AC gene and protein expression in NCI-H292 cells, and it could activate NF-κB and AP-1. Telithromycin significantly attenuated NTHi-induced MUC5AC mRNA and protein expression, and inhibit AP-1 activation as well, but not NF-κB.CONCLUSION: Telithromycin can attenuate NTHi-induced mucus expression via inhibition of AP-1 but not NF-κB.

Key words: nontypeable haemophilus influenzae, telithromycin, activator protein-1, NF-κB, MUC5AC

CLC Number:

R965.2

YANG Fan, LI Jian, GU Bin, ZHAO Wen-jian, YANG Zhi-ying. Telithromycin suppresses nontypeable Haemophilus influenzae-induced MUC5AC expression via inhibition of activator protein-1 signaling[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(2): 145-149.

References

[1] Kyd JM, McGrath J, Krishnamurthy A. Mechanisms of bacterial resistance to antibiotics in infections of COPD patients[J]. Curr Drug Targets, 2011,12(4):521-530.
[2] Fernaays MM, Lesse AJ, Sethi S, et al. Differential genome contents of nontypeable Haemophilus influenzae strains from adults with chronic obstructive pulmonary disease[J]. Infect Immun, 2006,74(6):3366-3374.
[3] Kohri K, Ueki IF, Shim JJ, et al. Pseudomonas aeruginosa induces MUC5AC production via epidermal growth factor receptor[J]. Eur Respir J, 2002,20(5):1263-1270.
[4] Vareille M, Kieninger E, Edwards MR, et al. The airway epithelium: soldier in the fight against respiratory viruses[J]. Clin Microbiol Rev, 2011,24(1):210-229.
[5] Escande F, Aubert JP, Porchet N, et al. Human mucin gene MUC5AC: organization of its 5'-region and central repetitiveregion[J]. Biochem J, 2001,358(Pt 3):763-772.
[6] Kratzer C, Graninger W, Macfelda K, et al. Comparative activities of antibiotics against intracellular non-typeable Haemophilus influenzae[J]. Wien Klin Wochenschr, 2007,119(9/10):297-302.
[7] Leiva M, Ruiz-Bravo A, Moreno E, et al. Telithromycin inhibits the production of proinflammatory mediators and the activation of NF-kappaB in in vitro-stimulated murine cells[J]. FEMS Immunol Med Microbiol, 2008,53(3):343-350.
[8] Song KS, Yoon JH, Kim KS, et al. c-Ets1 inhibits the interaction of NF-kappaB and CREB, and downregulatesIL-1beta-induced MUC5AC overproduction during airway inflammation[J]. Mucosal Immunol, 2012,5(2):207-215.
[9] Lim JH, Kim HJ, Komatsu K, et al. Differential regulation of Streptococcus pneumoniae-induced human MUC5AC mucin expression through distinct MAPK pathways[J]. Am J Transl Res, 2009,1(3):300-311.
[10] Morinaga Y, Yanagihara K, Miyashita N, et al. Azithromycin, clarithromycin and telithromycin inhibit MUC5AC induction by Chlamydophila pneumoniae in airway epithelial cells[J]. Pulm Pharmacol Ther, 2009,22(6):580-586.
[11] Ishimoto H, Mukae H, Sakamoto N, et al. Different effects of telithromycin on MUC5AC production induced by human neutrophil peptide-1 or lipopolysaccharide in NCI-H292 cells compared with azithromycin and clarithromycin[J]. J Antimicrob Chemother, 2009,63(1):109-114.
[12] Sugiyama K, Shirai R, Mukae H, et al. Differing effects of clarithromycin and azithromycin on cytokine production by murine dendritic cells[J]. Clin Exp Immunol, 2007,147(3):540-546.

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