Abstract: AIM: To investigate the expression and significance of Topo Ⅱ, P-gp, MRP, LRP, TS and other resistance genes in the gastrointestinal adenocarcinoma, in order to provide a theoretical basis for the choice of chemotherapeutic drugs. METHODS: The study included 25 cases of gastrointestinal cancer cases as experimental group, each case adjacent tissues as a control group, we detected with pathology image analysis, immunohistochemistry SP method and literature review. RESULTS: Topo Ⅱ positive rate in moderately differentiated adenocarcinoma tissues was higher than poorly differentiated adenocarcinoma. P-gp, MRP1, MRP3, TS, GST-π, β-Tubulin Ⅲ in different types of gastric cancer and colorectal cancer have different levels of expression in cancer tissues were higher than in the adjacent tissues. MRP and β-Tubulin Ⅲ in Mucinous adenocarcinoma have higher rate. LRP rarely expressed in the gastrointestinal adenocarcinoma. CONCLUSION: TopoⅡ cancer drug target is one of the positive rate of the corresponding positive effects of chemotherapy drugs. Combined detection of P-gp, MRP1, MRP3, TS, GST-π, β-Tubulin Ⅲ in the gastrointestinal adenocarcinoma of clinical medicine has a certain reference value. The group of selected genes in colorectal and gastric carcinoma were in a similar, There was no significant difference.

Key words: gastric adenocarcinoma, colon adenocarcinoma, Topo Ⅱ, resistance genes, IHC