Effect of gap junction in ischemic post-conditioning on cerebral ischemic-reperfusion injury in rats and its associated mechanism

Abstract

Abstract: AIM: To investigate the effect of gap junction in ischemic post-conditioning on cerebral ischemic-reperfusion injury in rats. METHODS: Sixty adult male SD rats were randomly divided into sham group, I/R group, IPO group, I/R+CBX group and IPO+CBX group. Thread occlusion method was used to make MCAO model. Neurological scores of rat were evaluated by Longa' score. Infarct volume of brain tissue was measured by TTC staining. Histopathology of cerebral tissue was detected by HE staining. The expressions of Cx43 and PKC protein were detected by Western Blot. RESULTS: In I/R group, neurological deficit scores decreased, cerebral infarction area increased and histopathology changed significantly, while similar changes did not found in sham group. IPO has protective effect on I/R injury. Similarly, CBX increased the protection of IPO on I/R injury obviously. Results of Western Blot showed, in I/R group, Cx43 protein increased significantly and PKC decreased compared to the sham group (P<0.01). Meanwhile, in IPO group Cx43 protein decreased extremely and PKC increased compared to I/R group. Similarly, in IPO+CBX group Cx43 protein decreased significantly and PKC increased compared to IPO group. CONCLUSION: IPO has protective effect on I/R injury through the gap junction, which may be associated with the change of PKC protein.

Key words: ischemic post-conditioning, PKC, Cx43, gap junction

CLC Number:

R965.2

JIAO Hao, TONG Xu-hui, DONG Shu-ying, GU Yu-chen, YU Bin-bin, YU Li. Effect of gap junction in ischemic post-conditioning on cerebral ischemic-reperfusion injury in rats and its associated mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(8): 841-845.

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