Study of GABAergic neurons electrophysiological changes after levetiracetam intervention in the epileptic mouse hippocampal

Abstract

Abstract: AIM: To study electrophysiological changes of GABAergic neurons of epileptic mouse in CA1 region of hippocampal and changes after levetiracetam intervention.METHODS: VB-Tg small mice were randomly divided into control group, model group, and levetiracetam group. Levetiracetam was intragastrically administered to mice in the levetiracetam group. After successfully establishing the mode.The tissue blocks of hippocampus were quickly isolated. Slices were held in oxygenated artificial cerebrospinal fluid. Sequential spike patterns (inter-spike intervals) and intrinsic properties (ARP;Vts;Vr) of GABAergic neurons were recorded by whole-cell clamp and were inputted into pClamp 9 for data acquisition.RESULTS: Compared with control group, absolute refractory period prolonged and threshold potentials increased and inter-spike interval (ISI) extended(P<0.05)in model group, the difference was statistically significant. Compared with model group, absolute orefractory periods obviously shortened(P<0.01)and threshold potentials significantly decreased(P<0.01)and and inter-spike interval (ISI) shortened(P<0.01)in levetiracetam group. after levetiracetam intervention, the difference was statistically significant.CONCLUSION: After levetiracetam intervention,the spike encoding and intrinsic properties of GABAergic neurons were raised. The functional enhancement of GABAergic neurons may play an important role in epileptic control.

Key words: levetiracetam, hippocampus, GABAergic neuron, epilepsy, action potential

CLC Number:

R965.2

NI Hong, ZHENG Bu-zhen, WANG En, JIN Xiao-ping, MA Chuan-hua, KE Shao-fa, ZHOU Yuan-lin, CHU Zhao-hu. Study of GABAergic neurons electrophysiological changes after levetiracetam intervention in the epileptic mouse hippocampal[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(5): 505-509.

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