Determination of caspofungin in human plasma by UPLC-MS/MS

Abstract

Abstract:

AIM: To establish a method to determine the concentration of caspofungin in human plasma by UPLC-MS/MS. METHODS: Plasma samples were spiked with roxioxithromycin as the internal standard and precipitated by methanol. The column was Ultimate AQ C₁₈ (100 mm×2.1 mm,3 μm) with the column temperature of 40 ℃. The mobile phase was acetonitrile and aqueous solution (5 mmol/L ammonium acetate, 0.2% formic acid), and the pump flow rate was 0.4 mL/min. The ion transitions were performed under ESI positive MRM model at m/z 547.5→538.2 for caspofungin and m/z 838.6→157.9 for internal standard. The analysis time was 6.5 min. RESULTS: Calibration curves of caspofungin showed good linear regression in the range of 0.1-25 μg/mL (R ²=0.999 5). The lowest quantification limit of caspofungin was 0.1 μg/mL. The extraction recovery was about 59.08%. The intra-day and inter-day relative standard deviation (RSD) were less than 15%. CONCLUSION: The established method is simple, accurate, sensitive and had good reproducibility, which can be used for the blood concentration determination of caspofungin.

Key words: caspofungin, UPLC-MS/MS, therapeutics drug monitoring

CLC Number:

R969

SHAO Hua, HE Zijian, HU Linlin, ZHAN Ying, HU Rongrong, HE Jie. Determination of caspofungin in human plasma by UPLC-MS/MS[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(10): 1133-1137.

References

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