MiR-200a alleviates renal interstitial fibrosis by inhibiting Wnt/β-catenin signaling pathway

Abstract

Abstract:

AIM: To investigate the inhibition of miR-200a on Wnt/β-catenin signaling pathway in unilateral ureteral obstruction mouse, and the effects of miR-200a on renal interstitial fibrosis. METHODS: Models of renal interstitial fibrosis were established by unilateral ureteral obstruction (UUO). Twenty-four six-weeks C57BL/6 male mice were randomly divided into four groups (n=6), Sham group, UUO group, UUO + miR-200a group and UUO + NC group. From day 1, UUO + miR-200a group was given miR-200a (i.v. by tail vein, 40 mg/kg), while UUO + NC group was given Negative Control (i.v. by tail vein, 40 mg/kg). Both groups were administered for three consecutive days. On day 7, HE and Masson staining were used to detect pathological changes in kidneys; RT-PCR and Western blot were used to detect mRNA and protein expressions of Wnt4, β-catenin, Fibronectin and α-SMA. RESULTS: Compared with Sham group, UUO group exhibited obvious renal tubular damage and interstitial collagen deposition; expressions of Wnt4, β-catenin, Fibronectin and α-SMA mRNAs and proteins were also increased in UUO group（P<0.05）. Compared with UUO group, UUO+miR-200a group presented minor renal tubular injury and interstitial collagen deposition; expressions of Wnt4, β-catenin, Fibronectin α-SMA mRNAs and proteins were also reduced in UUO+miR-200a group（P<0.05）. CONCLUSION: miR-200a can alleviate renal interstitial fibrosis by inhibiting the Wnt/β-catenin signaling pathway.

Key words: miR-200a, Wnt/β-catenin signaling pathway, renal interstitial fibrosis

CLC Number:

R965.2

CHEN Hua, GONG Yi, ZHOU Baoshang, ZHANG Jing. MiR-200a alleviates renal interstitial fibrosis by inhibiting Wnt/β-catenin signaling pathway[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(4): 381-386.

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