Analgesic effect and mechanism of the active component of cobras venom in southern Anhui province

doi:10.12092/j.issn.1009-2501.2018.11.006

Abstract

Abstract:

AIM: To explore the effect of topical analgesia of cobra venom analgesic factor (CVAF) in southern Anhui province and its possible mechanism. METHODS: Male SD rats were divided into normal saline control group (NS group), complete Freund's adjuvant pain group (CFA group), diclofenac sodium analgesic positive control group (CFA+SL group), flurbiprofen analgesic positive control group (CFA+FB group), CVAF analgesia group (CFA+CVAF group). The analgesic effect of CVAF gel, the active component of cobra venom was observed and compared. The pain thresholds of rats in each group were measured with a thermal tingling tester and mechanical tenderness tester. The content of IL-1 and TNF-α in the homogenate of spinal cord in each group was detected by using ELISA, the pathological changes of rat toe tissue was observed by using HE staining.RESULTS:Compared with CFA group, the weight loss of CVAF analgesia rats on the 7th and 14th day after administration was improved (P<0.05, P<0.01). The mechanical pain threshold and the thermal pain threshold of the CVAF group were all improved (P<0.01). The concentrations of IL-1 and TNF-α in the homogenate of spinal cord were relatively low (P<0.01). In HE staining, the toe tissue edema in the CVAF group was significantly reduced, and the inflammatory infiltration was relatively insignificant. CONCLUSION: The CVAF gel, the active ingredient of Cobra venom in southern Anhui province, can improve the inflammatory pain in rats hot pain threshold and mechanical pressure threshold; its mechanism may be related to reduce the release of inflammatory mediators, inhibiting the inflammatory response.

Key words: cobra venom, gel preparation, analgesia, anti-inflammatory

CLC Number:

R965.2

YU Ling，ZHANG Genbao，SUN Shuping. Analgesic effect and mechanism of the active component of cobras venom in southern Anhui province[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(11): 1235-1239.

References

［1］付四海, 冯梅, 熊燕.蛇毒多肽的新药研发进展［J］. 中南大学学报(医学版), 2017, 42 (11): 1334-1340.
［2］蒋世强, 潘能庆, 鄢航,等.眼镜蛇神经毒素研究进展［J］.现代农业科技, 2017, 17(4): 255-256.
［3］丁晓兰, 孙美玲, 秦正红.中华眼镜蛇毒口服给药镇痛作用的研究［J］. 抗感染药学,2013,10(3): 180-183.
［4］饶荣,杨祥良,刘卫.多肽蛋白质药物口服纳米载药系统研究进展［J］.医药导报,2018,37(6):697-702.
［5］赵子佳, 边学峰, 李晶峰, 等.蛋白肽类药物及其给药途径研究进展［J］.吉林中医药,2018,38(2): 188-190.
［6］闵志雪, 黄璐, 孙瑶, 等.皖南地区眼镜蛇毒活性组分镇痛效应的初步研究［J］.中国病理生理杂志, 2013, 29 (1): 150-154.
［7］Bráz JM, Sharif-Naeini R, Vogt D, et al. Forebrain GABAergic neuron precursors integrate into adult spinal cord and reduce injury-induced neuropathic pain ［J］. Neuron, 2012, 74 (4): 663-675.
［8］Woolf CJ, Salter MW. Neuronal plasticity: increasing the gain in pain ［J］. Science, 2000, 288 (5472): 1765-1769.
［9］Cirino G, Distrutti E, Wallace JL.Nitric oxide and inflammation［J］. Inflamm Allergy Drug Targets, 2006, 5 (2): 115-119.
［10］Miller RE, Miller RJ, Malfait AM.Osteoarthritis joint pain:The cytokine connection［J］.Cytokine, 2014 (70) :185-193.
［11］Stephens K，Cooper BA，West C，et al.Associations between cytokine gene variations and severe persisten breast pain in women following breast cancer surgery［J］. J Pain, 2014, 15 (2) :169-180.
［12］Finco D, Grimaldi C, Fort M, et al.Cytokine release assays:Current practices and future directions［J］.Cytokine, 2014, 66 (2):143-155.
［13］Lee AS, Ellman MB, Yan DY, et al.A current review of molecular mechanisms regarding osteoarthritis and pain［J］.Gene, 2013, 527 (2) :440-447.
［14］Subang MC, Richardson PM.Influence of injury and cytokines on synthesis of monocyte chemoattractant protein-1 mRNA in peripheral nervous tissue ［J］. Eur J Neurosci，2001, 13 (3): 521-528.
［15］Youn DH, Wang H, Jeong SJ. Exogenous tumor necrosis factor-alpha rapidly alters synaptic and sensory transmission in the adult rat spinal cord dorsal horn ［J］. J Neurosci Res，2008, 86 (13): 2867-2875.
［16］王海华, 张根葆, 闵志雪, 等.皖南地区眼镜蛇毒镇痛组分对大鼠炎性痛作用机制探讨［J］.中国临床药理学与治疗学, 2013, 18 (10): 1093-1099.

[1]	MAO Jun, RONG Yu, LI Yangen, XIONG Dengxi, ZHAO Peng, ZHA Zhengjiang. Effect and safety of flurbiprofen axetil in the analgesic treatment of patients with craniocerebral injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(9): 1056-1060.
[2]	SU Yang, ZHOU Feng, DING Jinlei. Effects of esketamine combined with sufentanil on postoperative analgesia and emotion after thoracoscopic radical resection of lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(1): 59-65.
[3]	WANG Xin, SUN Heliang, ZHANG Qingwei, LIU Cunming, WANG Zhongyun, YANG Chun. Postoperative analgesic effect of low-dose esketamine in patients with thoracoscopic lobectomy [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(9): 998-1003.
[4]	LI Yongliang, LIN Haiyan, ZHANG Lingling, DONG Yilong. Effects of preoperative dezocine patient-controlled analgesia pump on stress response and cognitive function in elderly patients with lower extremity fractures [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(3): 302-306.
[5]	WU Xiaoxia, ZHOU Hu. Application of remifentanil in postoperative ICU sedation and analgesia in patients with severe craniocerebral trauma [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(10): 1119-1124.
[6]	DENG Ying, BAI Shutong. Research advance of chrysoeriol on its pharmacological action and underlying mechnism [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(10): 1155-1162.
[7]	PENG Jing, SONG Jing, LUAN Jiajie. Mesenchymal Stem Cells: a new discovery in the treatment of novel coronavirus pneumonia [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(9): 1073-1079.
[8]	QI Qin, HE Xiang, WANG Gang, NA Sha, LV Lei, CHEN Guangliang. Anti-inflammatory and analgesic effects of total saponin of Dioscorea [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(3): 257-264.
[9]	ZHANG Shengpeng, MIU Dongdong, LIU Dingfeng, ZHU Xiaohong, ZHANG Chao, CHEN Yunyu, LIU Xiaoping. Hepatic toxicity and pharmacodynamic effects of paracetamol and chlorphenamine maleate with three herb extracts capsules [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(5): 489-495.
[10]	WANG Jun, JIANG Zongming, ZHENG Jungang, CAO Gang, HUANG Changshun, LU Zihui, JI Yiqin. Effects of oxycodone hydrochloride combined with dexmedetomidine postoperative analgesia on sleep quality of patients with gastrointestinal tumors [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(2): 165-169.
[11]	WANG Di, ZHANG Huanhuan, FANG Jie, ZHONG Yushen, YU Chenhuan. Effects of limoninon on LPS-induced acute lung injury in mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 8-12.
[12]	HUANG Hai, LIU Changjiang, JIN Xiaoyue. Mechanism of ethanolic extract of root of Anacyclus pyrethrum on two models of inflammation [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 47-50.
[13]	LENG Xiaowei, Rita, LI Min, ZHANG Tong, REN Liqun, FAN Zhimin. Comparison of pharmacodynamics of Fufang lujiao rutongning capsule and its original formula [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(8): 852-858.
[14]	HE Jianguo, YUAN Liyong, CHEN Lin, JIANG Juan. Preemptive analgesia effect of parecoxib sodium on children undergoing laparoscopic appendectomy [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(6): 705-708.
[15]	CHANG Yongli, XU Yinhong, WEN Xuemin, GUO Zhennan, ZHANG Lihui, HAN Ying, GUO Xiaoshu. Effect of preemptive analgesia with dezocine+ketorolac tromethamine complex on postoperative pain thresholds and inflammatory reaction in rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(3): 267-271.