Effects of bufalin on radiosensitivity of esophageal cancer Kyse150 cell line

doi:10.12092/j.issn.1009-2501.2018.07.002

Abstract

Abstract:

AIM: To investigate the radio-sensitivity effect of bufalin in Kyse150 cell line. METHODS: CCK-8 method was used to determine the IC₅₀ value of bufalin in Kyse150 cell line. The linear-quadratic (L-Q) curve was fitted according to the results of cloning formation and calculated the survival fraction of 2Gy (SF2) and sensitization enhancement ratio (SER). Annexin V-FITC/PI double staining and Western blot were used to detect the effect of bufalin on apoptosis of esophageal cancer Kyse150 cell line. The effect of different bufalin doses on Kyse150 cell line radiosenstivity was detected by cloning formation assay. RESULTS: CCK-8 results showed that the IC₅₀ value of bufalin in the Kyse150 cell line was 129.1 nmol/L (95% CI: 105.2-158.6). The L-Q curve fitted by colony formation assay showed that bufalin can significantly reduce the colony forming efficiency of cells after radiotherapy, and the sensitization enhancement ratio of bufalin in Kyse150 cell line was 1.463. Annexin V-FITC/PI double staining and Western blot results showed that bufalin can improve radiation-induced apoptosis, and showed that bufalin has a dose-dependent effect on radiosensitization of esophageal cancer Kyse150 cell line. CONCLUSION:Bufalin can enhance the radiosensitivity effect of the Kyse150 cell line in a dose-dependent manner.

Key words: bufalin, esophageal cancer, radiosensitivity, linear quadratic model, sensitization enhancement ratio

CLC Number:

R73
R965.2

FANG Weiyang, LI Yue, SHENG Lei, CUI Zhenzhen, ZHONG Fei, TONG Zhuting. Effects of bufalin on radiosensitivity of esophageal cancer Kyse150 cell line[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(7): 728-733.

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