Transcriptomic analysis of neonatal mammalian vestibular after hair cell injury by neomycin

doi:10.12092/j.issn.1009-2501.2019.05.001

Abstract

Abstract:

AIM: Vestibular hair cell damage is an important cause of vestibular dysfunction. This study tried to explore the changes of gene expression, signal pathway and biological process in the process of hair cell damage, so as to provide new ideas for hair cell regeneration and vestibular function recovery. METHODS: In the present study, RNA from the newborn mouse utricles was collected for RNA-seq immediately after the damage of neomycin, in order to investigate gene expression changes. RESULTS:There were 172 and 232 genes that were found to be up-regulated and down-regulated in neomycin-treated utricles. GO and KEGG pathway analyses of these genes revealed many deregulated cellular components, molecular functions, biological processes and signaling pathways that may be related to hair cell development.CONCLUSION: These results provide valuable reference and preliminary basis for future studies, and find that Wnt, Notch TGFβ and other signaling pathways that may be closely related to vestibular hair cell injury and regeneration, which may provide important signaling pathways and gene targets for vestibular hair cell regeneration research and clinical vertigo prevention.

Key words: vestibular hair cell, neomycin, RNA-seq

CLC Number:

R966

HAN Jinghong, LIU Yuchen, NIE Guohui. Transcriptomic analysis of neonatal mammalian vestibular after hair cell injury by neomycin[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(5): 481-489.

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