Paeoniflorin inhibits mast cell activation and degranulation through PI3K/AKT/m-TOR signaling pathway

doi:10.12092/j.issn.1009-2501.2019.09.001

Abstract

Abstract:

AIM: To study the effects of paeoniflorin on the activation and degranulation of mast cells (P815), and further to explore the mechanism. METHODS: The toxicity of paeoniflorin on P815 cells was evaluated by CCK-8 method. The effects of paeoniflorin on the release of histamine and β-HEX and the secretion of inflammatory factors IL-1β, IL-4, IL-8 and IL-12 were determined by ELISA method and chromogenic method. The effects of paeoniflorin on the expression of inflammatory factors and PARs were detected by qRT-PCR. Western blot was used to study the phosphorylation levels of PI3K, AKT and m-TOR. RESULTS: Paeoniflorin had no cytotoxicity to P815 cells when the concentration was 1, 10, 100 μg/mL, but effectively inhibited the release of histamine, β-HEX and IL-1β, IL-4, IL-8 and IL-12 after mast cells activation, and reduced the expression of PARs at the mRNA level. Additionally, paeoniflorin greatly reduced the phosphorylation levels of PI3K, AKT, m-TOR in mast cells treated with agonists. CONCLUSION: Paeoniflorin is an efficient inhibitor for mast cell activation and degranulation by blocking the PI3K/AKT/m-TOR signalling pathway.

Key words: paeoniflorin, mast cell, degranulation, allergic reaction

CLC Number:

R965.2

YU Jiaojiao, YANG Yang, LIU Ying, GUO Xiaoxi, XU Tianrui, AN Shu. Paeoniflorin inhibits mast cell activation and degranulation through PI3K/AKT/m-TOR signaling pathway[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(9): 961-968.

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