Lipoxin A4 attenuates hypoxia induced oxidative stress and apoptosis in human first trimester trophoblast cells

Abstract

Abstract:

AIM: To investigate the effect of lipoxin A₄ (LXA₄) on oxidative stress and apoptosis of human first trophoblast cells induced by hypoxia. METHODS: Primary human trophoblast cells were randomized into normoxia group, hypoxia group and hypoxia+LXA₄ (1,10 and 100 nmol/L) groups. The level of ROS was determined by DCFH-DA, the activity of SOD, CAT and GPx were detected by the corresponding kits. The apoptosis rate was analyzed by flow cytometer method and the level of Bcl-2 and Bax protein were observed by Western Blot. RESULTS: Compared with normoxia group, the level of ROS and apoptosis rate increased (P<0.05) while the activity of SOD, CAT and GPx decreased (P<0.05) in hypoxia group. Compared with hypoxia group, the level of ROS and apoptosis rate decreased (P<0.05), while the activity of SOD, CAT and GPx increased (P<0.05) in hypoxia+LXA₄ (1,10 and 100 nmol/L) groups. Compared with normoxia group, the level of Bcl-2 protein in hypoxia group decreased and the level of Bax protein increased (P<0.05). Compared with hypoxia group, the level of Bcl-2 protein in hypoxia+LXA₄(1,10 and 100 nmol/L) groups increased and the level of Bax protein decreased (P<0.05). CONCLUSION: Lipoxin A₄ attenuates hypoxia induced oxidative stress and apoptosis in human first trophoblast cells. The regulation of Bcl-2/Bax expression may be an important mechanism of LXA₄ in inhibiting apoptosis.

Key words: lipoxin, trophoblast, hypoxia, oxidative stress, apoptosis

CLC Number:

R965.2

HUANG Yanjun, WU Jie, JIANG Wei, WANG Xiao, HUANG Yinping . Lipoxin A₄ attenuates hypoxia induced oxidative stress and apoptosis in human first trimester trophoblast cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(4): 361-366.

References

［1］Young BC, Levine RJ，Karumanchi SA.Pathogenesis of preeclampsia［J］. Annu Rev Pathol, 2010, 5(2):173-192.
［2］Romano M. Lipoxin and aspirin-triggered lipoxins［J］. Scientific World J, 2010, 10:1048-1064.
［3］Lin F, Zeng P, Xu Z, et al. Treatment of Lipoxin A4 and its analogue on low-dose endotoxin induced preeclampsia in rat and possible mechanisms［J］. Reprod Toxicol, 2012, 34(4):677-685.
［4］Serhan CN. Lipoxins and aspirin-triggered 15-epi-lipoxin biosynthesis: an update and role in anti-inflammation and pro-resolution［J］. Prostaglandins Other Lipid Mediat, 2002, 68-69:433-455.
［5］Siddiqui IA, Jaleel A, Tamimi W, et al. Role of oxidative stress in the pathogenesis of preeclampsia［J］. Arch Gynecol Obstet, 2010, 282(5):469-474.
［6］Tannetta D, Sargent I. Placental disease and the maternal syndrome of preeclampsia: missing links ［J］ ?Curr Hyperten Rep, 2013, 15(6):590-599.
［7］Burton GJ, Jauniaux E. Oxidative stress［J］. Best Pract Res Clin Obstet Gynaecol, 2011, 25(3): 287-299.
［8］Serdar Z, Gür E, Colakoethullary M, et al. Lipid and protein oxidation and antioxidant function in women with mild and severe preeclampsia［J］. Arch Gyneeol Obstet, 2003, 268(1):19-25.
［9］Vanderlelie J, Venardos K, Clifton VL, et al. Increased biological oxidation and reduced anti- oxidant enzyme activity in pre-eclamptic placentae［J］. Placenta, 2005, 26(1):53-58.
［10］Cindrova-Davies T, Spasic-Boskovic O, Jauniaux E, et al. Nuclear factor-kappa B, p38, and stress-activated protein kinase mitogen-activated protein kinase signaling pathways regulate proinflammatory cytokines and apoptosis in human placental explants in response to oxidative stress: effects of antioxidant vitamins［J］. Am J Pathol, 2007, 170(5):1511-1520.
［11］Huppertz B, Kingdom JC. Apoptosis in the trophoblast--role of apoptosis in placental morphogenesis［J］. J Soc Gynecol Investig, 2004,11(6):353-362.
［12］Redman CW, Sargent IL. Microparticles and immunomodulation in pregnancy and pre-eclampsia［J］. J Reprod Immunol, 2007, 76(1/2):61-67.
［13］Germain SJ, Sacks GP, Sooranna SR, et al. Systemic inflammatory priming in normal pregnancy and preeclampsia: the role of circulating syncytiotrophoblast microparticles［J］. J Immunol, 2007, 178 (9):5949-5956.
［14］王佳, 高峰, 张春兵,等. 川芎嗪降低咖啡因诱导的PC12细胞凋亡的研究［J］. 长春中医药大学学报, 2014, 30(1):14-17.
［15］金晓盛, 吴厉锋, 钱小英,等. 促红细胞生成素后处理对大鼠再灌注损伤肺细胞凋亡的干预及机制［J］. 中国临床药理学与治疗学, 2014, 19(4):371-376.
［16］周杰, 严凌, 黄多新. 线粒体融合素2通过Ras-PI3K-Akt信号途径抑制缺血再灌注诱导的乳鼠心肌细胞凋亡［J］. 中国临床药理学与治疗学, 2014, 19(9):973-979.
［17］Siddiqui WA, Ahad A, Ahsan H. The mystery of BCL2 family: Bcl-2 proteins and apoptosis: an update［J］. Arch Toxicol, 2015, 89(3):289-317.
［18］Kvansakul M, Hinds MG. The Bcl-2 family: structures, interactions and targets for drug discovery［J］. Apoptosis, 2015, 20(2):136-150.

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Lipoxin A₄ attenuates hypoxia induced oxidative stress and apoptosis in human first trimester trophoblast cells

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