Experimental study of benazepril intervene Wnt signaling inhibits renal fibrosis in UUO rats

Abstract

Abstract:

AIM: To investigate the antagonism mechanism of benazepril on renal fibrosis of unilateral uretreal obstructire(UUO). METHODS: Fifty-four Sprague-Dawley (SD) rats were randomly divided into three groups: sham operation group, model group, benazepril group. All the other groups were treated with surgery method of unilateral ureteral ligation to establish a rat model of renal fibrosis except the sham operation group. The pathological changes were observed by Masson staining; the positive staining expression of TGF-beta1, Col-IV, Wnt1, Wnt4 in frozen sections were observed by immunofluorescence staining; the expressions TGF-beta1, Col-IV, Wnt1and Wnt4 mRNA in renal tissues were detected by real time fluorescence quantitative PCR. RESULTS: Masson staining results: compared with the sham group, the parts of blue dye collagen fiber and the degree of renal fibrosis increased significantly in all modeled groups; compared with the model group, the parts of blue dye collagen fiber and the degree of renal fibrosis decreased significantly in benazepril group. Immunofluorescence and PCR results: compared with the model group, the positive expression of Wnt1, Wnt4, TGF-beta1, Col - IV and mRNA in the benazepril group decreased significantly. CONCLUSION: Benazepril can improve renal fibrosis by inhibiting the expression of TGF-beta1, Col-V, Wnt1, Wnt4 and ameliorating the level of renal fibrosis.

Key words: benazepril, renal interstitial fibrosis, unilateral ureteral obstruction, Wnt signaling

CLC Number:

R965.2

ZHAO Yanyun, WU Xiaoyan, WANG Guomin, FENG Yulin, DONG Feixia. Experimental study of benazepril intervene Wnt signaling inhibits renal fibrosis in UUO rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(12): 1332-1339.

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