Abstract:

AIM: To observe clinical the effects of Huashenzilu on mice models with acute and chronic eczema. METHODS: Eczema was induced by repeated local exposure to DNCB, and then the mice received Huashenzilu. Using ELISA kit method to test the serum concentration of IL-2, IL-4，TNF-alfa，IFN-gamma, and HE staining to test histopathological changes. The ear swelling and scratching index were measured, and ear allergy score were measured according to the standard for evaluation. RESULTS: After the treatment of Huashenzilu, the serum concentration of IL-4，TNF-alfa significantly increased and the serum concentration of IL-2，IFN-gamma significantly decreased in the chronic mice compared to the model group (P<0.01). The ear swelling, scratching index and ear allergy score were significantly decreased. The phenomenon of epidermal thickening, acanthosis layer cavitation, and mast cells, macrophages infiltration were improved compared to the model group. CONCLUSION: Huashenzilu can control the chronic eczema in mice through the mechanism of regulating the balance between Th1 and Th2 immune response.

Key words: Huashenzilu, chronic eczema, immune response