Immunological pathology mechanism research of cervical cancer based on tumor related macrophage

Abstract

Abstract:

AIM:To analyze the mechanism of tumor-associated macrophage (TAMs) in the development of cervical cancer and to investigate its correlation with Th1/Th2 and Th17/CD4+CD25+Foxp3+Treg. METHODS:Twenty seven cases of cervical cancer and 53 cases of cervical intraepithelial neoplasias (including 22 cases of CINⅡ and 31 cases of CINⅢ) were selected as subjects. The venous blood of patients before treatment was extracted to detect Th1/Th2 and Th17/CD4+CD25+Foxp3+Treg with flow cytometry, and detect serum IFN-γ, IL-4, IL-17A, IL-17F, TGF-β1 and IL-10 levels with ELISA Kits. Furthermore, pathological tissues were extracted during operation, and its TAMsCD68 expression was detected by immunohistochemistry technique. RESULTS:The Th1/Th2 and Th17/CD4+CD25+Foxp3+Treg of cervical cancer were both lower than those of CINⅢ, and those of CINⅢ were both lower than CINⅡ, the difference between groups had statistical significance (P<0.05). The serum IFN-γ, IL-4, IL-17A, IL-17F, TGF-β1 and IL-10 levels of cervical cancer were all higher than those of CINⅢ, and those of CINⅢ were all higher than CINⅡ, the difference between groups had statistical significance (P<0.05). The TAMsCD68 expression level of cervical cancer was higher than that of CINⅢ, and that of CINⅢ was lower than CINⅡ, the difference between groups had statistical significance (P<0.05). The correlation analysis results showed TAMsCD68 expression level had negative correlations with Th1/Th2, Th17/CD4+CD25+Foxp3+Treg, and serum IL-17A level, whereas presented positive correlations with serum IL-10 and IL-4 level (P<0.05). CONCLUSION:TAMs is closely related with Th1/Th2 and Th17/CD4+CD25+Foxp3+Treg in cervical cancer, and possibly is mediating the occurrence and development of cervical cancer through influencing the balance of these two systems.

Key words: tumor-associated macrophage, cervical cancer, Th1/Th2, Th17/CD4+CD25+Foxp3+Treg

CLC Number:

R737.3

CHEN Xing, WU Weiling, CHEN Shanshan, YE Liya, MEI Shuangshuang, WANG Jing, HE Yu, CHAI Zeying. Immunological pathology mechanism research of cervical cancer based on tumor related macrophage[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(12): 1394-1399.

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