Expression of absent in melanoma 2 in acute pancreatitis of mice model

doi:10.12092/j.issn.1009-2501.2018.01.010

Abstract

Abstract:

AIM: To explore the expression of absent in melanoma 2 (AIM2) in pancreas in acute pancreatitis (AP). METHODS: Caerulein was used to set AP mice model, which were then divided into the control group and the AP group. Q-PCR and Western blot were used to detect AIM2, IL-1beta and IL-18 mRNA and protein expression in peripheral blood mononuclear cells (PBMCs). ELISA method was used to detect the concentration of IL-1beta and IL-18 in culture supernatants of PBMCs. RESULTS: Q-PCR and Western blot showed that compared with the control group, the expression of AIM2, IL-1β and IL-18 mRNA and protein in PBMCs AP group increased significantly (P<0.01). ELISA test results showed that compared with the control group, the concentration of IL-1beta and IL-18 from culture supernatants of PBMCs in AP group increased significantly (P<0.01). CONCLUSION: The higher expression of AIM2 in PBMCs of AP showed activation of the AIM2 inflammasome in PBMCs results in exacerbated IL-1beta and IL-18 production. Therefore, AIM2 might be a potential therapeutic target in clinical AP treatment.

Key words: acute pancreatitis, absent in melanoma 2, inflammasome

CLC Number:

R965.2

YANG Feiyun, ZHU Xiuying, ZHANG Jianxin, YANG Yaqin. Expression of absent in melanoma 2 in acute pancreatitis of mice model[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 51-54.

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