Protective effects of Baicalin to the human melanocyte cocultured with H2O2

doi:10.12092/j.issn.1009-2501.2018.04.003

Abstract

Abstract:

AIM: To study the mechanism of anti-oxidant and melanocyte protection by Baicalin. METHODS: Different concentration of Baicalin and H₂O₂ were cocultured with normal human melanocyte. Cell proliferation was detected by cell counting kit-8 assay, morphology of the human melanocyte was observed by ordinary inverted microscope, and LDH assay was used to detect cell viability. For mechanism research, DCFH-DA ROS level was assayed by flow cytometry. Induced HSP70 from melanocyte was detected using HSP70 high sensitivity ELISA kit. By RT-PCR, mRNA levels of CAT and GPX1 were dectected. RESULTS: Baicalin has obvious protective effects on melanocytes damage caused by H₂O₂. Compared with control group,in Baicalin groups, cell shape was changed and increased. In 12.5, 25, 50 μmol/L Baicalin groups, the effects of on melanocyte proliferation rate were higher than the control group (P<0.05). Baicalin inhibited the expression of LDH in black pigment cells in a dose-dependent manner, there was statistically significant difference (P<0.05). After H₂O₂ oxidative damage of melanocytes, Baicalin could significantly promote the expression of CAT and GPX1 mRNA, and the secretion of HSP70 was significantly increased. CONCLUSION: Baicalin protects human melanocytes from oxidative damage by inhibiting H₂O₂-induced apoptosis. It is referential for the treatment of vitiligo.

Key words: Baicalin, melanocyte, oxidative damage, vitiligo

CLC Number:

R965.2

ZHU Yiping, JIN Rong, LIN Fuquan, ZHOU Miaoni, WANG Suiquan, XU Aie. Protective effects of Baicalin to the human melanocyte cocultured with H₂O₂[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(4): 377-382.

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Protective effects of Baicalin to the human melanocyte cocultured with H₂O₂

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