Table of Content

Volume 23 Issue 4
26 April 2018

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Effects of Astragalus-Angelica combination on VSMC proliferation of vascular intimal hyperplasia in rats

YAN Huifang, XU Hao, PENG Xiwei, ZHU Jiahuan, DENG Changqing

2018, 23(4):  361-369.  doi:10.12092/j.issn.1009-2501.2018.04.001

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AIM: To investigate the effects of different Astragalus-Angelica combination on vascular smooth muscle cells (VSMC) phenotype transformation, cell cycle and PI3K/AKT signal pathway of vascular intimal hyperplasia in rats. METHODS: A model of intimal hyperplasia of thoracoabdominal aorta was established by balloon catheter injury in male Sprague-Dawley rat, and the model rats were administrated Astragalus and Angelica with single use or different ratios for 14 days by means of intragastric administration. Then the thoracoabdominal aorta was taken out to analyze the pathological changes of the VSMC, expression of cell cycle and PI3K/Akt signaling pathway related proteins in hyperplastic intima.  RESULTS: After balloon injury of aortic endothelium, VSMC phenotype transformation and proliferation can be induced, resulting in intimal hyperplasia. In hyperplastic intima, the expression of SM-actin can be enhanced and the expression of PCNA can be inhibited by the Astragalus and Angelica with single use and Astragalus-Angelica of 1∶1 and 5∶1 combination. The increased expression of cyclinD1 or cyclinE in damaged blood vessels and p-PI3K or p-Akt protein in hyperplastic intima can be inhibited by the Astragalus and Angelica with single use and Astragalus-Angelica of 1∶1 and 5∶1 combination. CONCLUSION: Vascular intimal hyperplasia can be inhibited by Astragalus-Angelica compatilibity. The mechanism may be related to the inhibition of PI3K/Akt signaling pathway activation, which lead to the inhibition of the phenotypic transformation and cell proliferation of VSMC, and then play the role of anti VSMC proliferation.

Study of Tongjing dingyunwan blocking NF-κB activation on the MCP-1/CCR2 signaling pathway and expressions of TNF-α, IL-1β, IL-6 in the cervical spondylosis rats

WANG Xianhai, LI Zhenbin, LI Xiaomei, ZHANG Jianguo, SHI Xinmin, XU Haiyan, FENG Minlu, SHI Mengqiong

2018, 23(4):  370-376.  doi:10.12092/j.issn.1009-2501.2018.04.002

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AIM: To investigate Tongjing dingyunwan blocking nuclear factor-κB (NF-κB) activation on the MCP-1/CCR2 signaling pathway and expressions of TNF-α, IL-1β, IL-6 in the cervical spondylosis rats.  METHODS: The cervical spondylosis model was established through operative method. Therapeutic groups were given Tongjing dingyunwan (5.0 g/kg and 10.0 g/kg) and and Jingfukang granule (0.84 g/kg), once a day for four weeks, the general condition of rat was observed. After oral administration of Tongjing dingyunwan for four weeks, blood viscosity, serum TNF-α, IL-1β, and IL-6 were measured; Cervical intervertebral disc morphology was analyzed by HE staining; Real-time PCR was applied to detect the mRNA expressions of MCP-1 and CCR2, protein expression of NF-κBp65 in intervertebral disk tissue were detected by western blotting. RESULTS: Tongjing dingyunwan (5.0 g/kg and 10.0 g/kg) and Jingfukang granule (0.84 g/kg) might significantly improve the general condition of the cervical spondylosis rats, decrease blood low shear viscosity, shear viscosity, viscosity value, serum TNF-α, IL-1β, IL-6 contents and the mRNA and protein expressions of MCP-1, CCR2 and NF-κBp65 in intervertebral disk tissue (P<0.05, P<0.01, respectively), alleviate the proinflammatory cytokine on intervertebral disc injury, reduce the height and degeneration of the cervical intervertebral disc, reduce the fracture of the fibrous ring, the nucleus pulposus, the number of cells and the thickness of the cartilage, especially Tongjing Dingyunwan 10.0 g/kg group. CONCLUSION: Tongjing dingyunwan has a better therapeutic effect on cervical spondylosis rats. Its mechanism is mainly related to reducing blood viscosity, inhibiting the MCP1/CCR2 signaling pathway, activation of NF-κB, and productions of TNF-α, IL-1β, IL-6.

Protective effects of Baicalin to the human melanocyte cocultured with H₂O₂

ZHU Yiping, JIN Rong, LIN Fuquan, ZHOU Miaoni, WANG Suiquan, XU Aie

2018, 23(4):  377-382.  doi:10.12092/j.issn.1009-2501.2018.04.003

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 AIM: To study the mechanism of anti-oxidant and melanocyte protection by Baicalin.  METHODS: Different concentration of Baicalin and H₂O₂ were cocultured with normal human melanocyte. Cell proliferation was detected by cell counting kit-8 assay, morphology of the human melanocyte was observed by ordinary inverted microscope, and LDH assay was used to detect cell viability. For mechanism research, DCFH-DA ROS level was assayed by flow cytometry. Induced HSP70 from melanocyte was detected using HSP70 high sensitivity ELISA kit. By RT-PCR, mRNA levels of CAT and GPX1 were dectected. RESULTS: Baicalin has obvious protective effects on melanocytes damage caused by H₂O₂. Compared with control group,in Baicalin groups, cell shape was changed and increased. In 12.5, 25, 50 μmol/L Baicalin groups, the effects of on melanocyte proliferation rate were higher than the control group (P<0.05). Baicalin inhibited the expression of LDH in black pigment cells in a dose-dependent manner, there was statistically significant difference (P<0.05). After H₂O₂ oxidative damage of melanocytes, Baicalin could significantly promote the expression of CAT and GPX1 mRNA, and the secretion of HSP70 was significantly increased. CONCLUSION: Baicalin protects human melanocytes from oxidative damage by inhibiting H₂O₂-induced apoptosis. It is referential for the treatment of vitiligo.

Effect of genistein on cartilage mediated by JAK2/STAT3 signaling pathway in rats with osteoarthritis

HU Jiong, WANG Weidong, WANG Changxing, DONG Liqiang, LIU Qiming

2018, 23(4):  383-388.  doi:10.12092/j.issn.1009-2501.2018.04.004

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AIM: To investigate the effect of genistein on cartilage and its relation to JAK2/ STAT3 signaling pathway in rats with osteoarthritis（OA）. METHODS: SD rats were randomly divided into control group, OA group and genistein with OA group. After 6 weeks of treatment, the histological morphology of cartilage specimen was observed by Histologic staining; the expression of TNF-α in synovial fluid of rats was detected by ELISA and the expressions of p-JAK2, p-STAT3, MMP-13, Bax and Bcl-2 in articular chondrocytes were detected by Western blot. RESULTS: Genistein can effectively increase the content of collagen and proteoglycan in the OA articular cartilage, and make the cartilage structure close to the normal cartilage. Compared with OA group, the expression of TNF-α in genistein+OA group was significantly down regulated (P<0.01), compared with the normal group, there was no significant difference in TNF-α expression of OA+genistein (P>0.05). Compared with OA group, the expression level of p-JAK2, p-STAT3 and Bcl-2 protein in genistein+OA group increased significantly (P<0.05), and the expression level of MMP-13 and Bax decreased significantly (P<0.05). CONCLUSION: JAK2/STAT3 signaling pathway is closely related to the pathological process of cartilage degeneration in osteoarthritis. Genistein can activate JAK2 STAT3 signaling pathway, inhibit the inflammation of osteoarthritis and the apoptosis of chondrocytes and relieve the degeneration of articular cartilage as well as reduce the level of OA progress.

Inhibition effects of Danhong injection on three transporters in human primary hepatocytes in vitro

ZHANG Jingxian, CHEN Tao, ZHANG Xiangqi, SHI Meizhi, CHEN Junjun, ZHANG Kezhi, YANG Jiao, GUO Cheng, WANG Laiyou, HAN Yonglong

2018, 23(4):  389-394.  doi:10.12092/j.issn.1009-2501.2018.04.005

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AIM: To study the inhibition effects of Danhong injection on NTCP, OATP and OCT1 in vitro. METHODS: The cells were divided into three groups: experimental group (different content of Danhong injection 0.03%, 0.06%, 0.12%, 0.25%, 0.50%, 1.00% and 2.00%), positive control group (cyclosporine A solution 10 μmol/L, rifamycin solution 200 μmol/L, verapamil solution 20 μmol/L) and blank control group (Hanks balance salt solution). One hundred and sixty microliters of working solution were incubated with human primary hepatocytes for 15 min and then mixed with 40 μL substrate (ox-gall sulfonic acid sodium 5 μmol/L, rosuvastatin 5 μmol/L and 1-methyl-4-phenyl pyridinium 5 μmol/L), respectively. Content of the substrate of cell lysis buffer was measured by liquid chromatography/tanderm mass spectrometry (LC-MS/MS) to determine the inhibition of Danhong injection towards transporters' activity and the inhibition effect were evaluated with 50% inhibition concentration (IC₅₀) values. RESULTS: Compared with the blank control group, OATP was inhibited significantly by the 0.12%, 0.25%, 0.50%,1.00% and 2.00% Danhong injection (P＜0.05, P＜0.01, P＜0.01, P＜0.01, P＜0.01). IC₅₀ values of Danhong injection on NTCP, OATP and OCT1 were ≥2.00%, 0.85% and ＞2.00%, respectively, and the value of 0.85% was between daily doses. CONCLUSION: Danhong injection show feeble inhibitory effect on NTCP and has no significant influence with OCT1, and may not lead to herb-drug interaction. But Danhong injection show strong inhibitory effect on OATP. Special attention should be paid to herb-drug interaction in drug combination to avoid possible adverse reaction.

Effects of inhibiting the expression of DNMT1 on the p16 gene of gastric cancer cells

ZHAO Jun, ZHU Lei, ZHAO Guohai

2018, 23(4):  395-399.  doi:10.12092/j.issn.1009-2501.2018.04.006

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AIM: To inhibit the expression of DNMT1 (DNA metylation transferase 1, DNMT1) gene in gastric cancer BGC-823 cells by RNAi and to investigate the expression of p16 gene after the silence of DNMT1.  METHODS: siRNA plasmid 1,2,3 and negative control siRNA plasmid b were constructed according to the nucleotide sequence of DNMT1. Gastric cancer BGC-823 cells were transfected with the constructed siRNA plasmid in mediation of liposome. Transcription levels of DNMT1 mRNA were determined by Q-PCR, protein expression levels by Western blot to select the best interference plasmid after the DNMT1 gene silence. Then used the same methods to investigate the expression of p16 gene in gastric cancer cells, and effects of inhibiting the expression of DNMT1 on the p16 gene of gastric cancer cells. RESULTS: The RNAi could effectively inhibit the expression of DNMT1 gene in gastric cancer BGC-823 cells. After transfection, transcription levels of DNMT1 mRNA were determined by Q-PCR, and translation levels of DNMT1 protein by Western blot. The results showed that the expression of DNMT1 in siRNA plasmid 2 group was markedly down-regulated compared to other transfection groups, negative control group and blank control group (P＜0.05); so siRNA plasmid 2 was the best interference plasmid. Used the same methods to investigate the expression of p16 gene in the best interference group (siRNA plasmid 2), negative control group and blank control group, the expression of p16 gene mRNA (1.6727±0.2242) and protein (0.9227±0.0337) in the best interference group was markedly up-regulated compared to the negative control group (1.0025±0.0877), (0.5440±0.0229) and blank control group (0.4729±0.0940), (0.4767±0.0774), (P＜0.05). CONCLUSION: RNAi can inhibit the DNMT1 gene expression to promote the de-methylation and restore expression of  p16 gene.

Lumbar plexus block with Ropivacaine for hind limbs pain of chronic ischemia in rats

FAN Yimin, WANG Limin, GUO Xiaoshu, FENG Gaizhuang, LI Xiuping

2018, 23(4):  400-405.  doi:10.12092/j.issn.1009-2501.2018.04.007

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AIM: To observe the Ropivacaine treatment with lumbar plexus block rat hind limbs pain of chronic ischemia.   METHODS: Lumbar plexus of Normal SD rats were separated to induce neural discharge. Effects of different concentrations of Ropivacaine (0.025%, 0.05%, 0.075%, 0.1% and 0.125%) on neural discharges were then observed to determine the best concentration. A total of 39 healthy SD healthy adult rats were randomly divided into the normal group, ischemic group and ischemia+block group（n=13）. Chronic skeletal muscle ischemia model were prepared with unilateral femoral artery cannula, block lumbar plexus. One week after ischemia, histamine and substance P of the rat peripheral blood were observed by ELISA, pain threshold was determined by hot plate method, gastrocnemius was observed in paraffin section through HE staining. RESULTS: 0.075% Ropivacaine was the best block concentration. The rat pain threshold was significant different between groups (P<0.05) except normal group vs. ischemia +block group; pain threshold of the ischemia+block group increased more significantly than the ischemia group. The rat serum histamine, substance P were significant different between groups (P<0.05); histamine and substance P of the ischemia+block group decreased more significantly than the ischemia group. CONCLUSION: Lumbar plexus block with Ropivacaine treatment can effectively alleviate ischemia pain in rats.

Antrodia camphorata polysaccharide improved neurobehavioral function in Parkinson mice via reducing the expression of NLRP3-Caspase1 inflammatory body

YANG Yi，GUAN Qiaobin，GUO Li，HAN Chenyang

2018, 23(4):  406-412.  doi:10.12092/j.issn.1009-2501.2018.04.008

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 AIM: To study the mechanism of Antrodia camphorata polysaccharide improved neurobehavioral function in Parkinson mice via reducing the expression of NLRP3-Caspase1 inflammatory body.  METHODS: The Parkinson model mice was constructed by 6-OHDA injection, and the mice model was successfully constructed by TH immunohistochemical staining and behavior intervention of Antrodia camphorata polysaccharide, neurobehavioral experiments were performed at four time points. The independent behavioral ability and coordination ability of mice were detected by rotating rod test and climbing rod test respectively. The peripheral blood of the tail vein of mice was collected at four time points and the expression of Caspase1 and IL-1β in peripheral blood was detected by ELISA method. After the 7-day intervention, the mice were killed and the brain tissue striatum was taken. Caspase1, pro Caspase1 and NLRP3 in striatum were detected by Western blot; monoamine neurotransmitters in striatum were determined by high performance liquid chromatography; mRNA expression of Caspase1, NLRP3, IL-1β, IL-4 and IL-6 were detected by RT-QPCR. NISSl staining was used to detect the apoptosis of neural cells in the brain tissue of mice. RESULTS: 6-OHDA injection could cause Parkinson like lesions in mice, and the expression of TH protein was down regulated significantly. The behavior of mice was significantly improved  after Antrodia camphorata polysaccharide intervention（P<0.05）; in striatum, the expression of Caspase1, proCaspase1, NLRP3 were significantly lower than those in model mice（P<0.05）; the mRNA expression of caspase1, NLRP3, IL-1 beta, IL-4 and IL-6 was down regulated（P<0.05）; the expression of monoamine neurotransmitters increased in striatum（P<0.05）. CONCLUSION: Antrodia camphorata polysaccharide improved neurobehavioral function in Parkinson mice via reducing the expression of NLRP3-Caspase1 inflammatory body. This may be one of the mechanisms for the treatment of Parkinson with Antrodia camphorata polysaccharide.

Study on the differences gene between proximal and distal colon cancer based on GEO database by bioinformatics

ZHANG Yiwen, LOU Qianwen, ZHONG Like, PAN Zongfu, FANG Luo, HUANG Ping

2018, 23(4):  422-427.  doi:10.12092/j.issn.1009-2501.2018.04.010

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AIM: To investigate the distinguish differences gene and its key pathways by analyzing the microarray data of the colon cancer patients with location information.  METHODS: Gene expression profiles of the colon cancer with the information of location information in GEO database were selected. Then, using the GO gene functional annotation and KEGG pathway enrichment analysis, screening can represent different characteristics of location of colon cancer gene cluster & pathway, and visualization analysis for protein interaction network. RESULTS: After bioinformatics analysis, it was found that location affected the biological characteristics of colon carcinoma, and significantly altered some important tumor signaling pathways, such as Wnt signaling pathway, extracellular matrix related pathways and immuno-regulatory pathways. CONCLUSION: This study employed bioinformatics method from various perspectives to define the gene expression characteristics of different location in colon carcinoma. These results may provide theoretical basis for accurate treatment of colon cancer.

Optimization of SAE reconciliation in clinical trail

ZHOU Bei, YU Hao

2018, 23(4):  428-433.  doi:10.12092/j.issn.1009-2501.2018.04.011

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AIM: To realize the semi-automation of SAE (serious adverse events) reconciliation between clinical database and safety database through SAS (statistic analysis system) program, reduce the manual work and improve the accuracy of reconciliation.  METHODS: A CRO company's SAE reconciliation was taken as example, the SAE of safety database was imported into SAS through Proc Import procedure, which was combined with SAE of clinical database and was sorted by subject, AE number or AE name; the same variable one by one was compared, located and marked the inconsistent data point in EXCEL by SAS DDE language, the consistency check was more targeted and more intuitive.RESULTS:The inconsistent data point was accurately located and the workload of manual check was reduced; meanwhile, screening and matching through SAS program reduced the random errors which manual operation may produce. CONCLUSION: This method can assist traditional manual check to a certain extent; also, it can reduce the human dependence, guarantee the quality of check and process a high value of practical application.

Effects of Yinaojieyu prescription on the kidney deficiency and liver depression of Parkinson's disease and the effect on DA, 5-HT and NE in serum

ZHOU Rong, LI Wei, HU Wanhua

2018, 23(4):  434-439.  doi:10.12092/j.issn.1009-2501.2018.04.012

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AIM: To investigate the clinical efficacy of Yinaojieyu prescription in the treatment of depression of kidney deficiency and liver depression type Parkinson disease, and to evaluate the effects of Yinaojieyu prescription on serum DA, 5-HT and NE.  METHODS: Sixty patients with depression of kidney deficiency and liver depression type Parkinson disease were divided into the study group and the control group, 30 cases in each group. The control group was treated with paroxetine tablets. The study group was treated with Yinaojieyu prescription. Hamilton Depression Scale (HAMD) was used to evaluate the depressive state of patients. The Unified Parkinson Disease Rating Scale (UPDRS) was used to assess the condition of Parkinson's disease in the patient. WHO quality of life scale (WHOQOL-BREF) was used to assess the quality of life of patients with made. The levels of serum dopamine (DA), 5-serotonin (5-HT) and norepinephrine (NE) were measured before and after treatment in the two groups. RESULTS: The curative effect of the study group was higher than that of the control group, but the difference was not statistically significant (P>0.05); HAMD score and UPDRS score of the two groups after treatment were significantly decreased, while WHOQOL-BREF scores increased significantly (P<0.05); HAMD score and UPDRS score of the study group after treatment were lower than those of the control group; WHOQOL-BREF score was higher than that of the control group (P<0.05); The levels of DA, NE and 5-HT in two groups after treatment were significantly higher than those before treatment (P<0.05); after treatment, DA, NE, 5-HT of the study group increased as compared with the control group (P<0.05).No significant difference was observed of incidence of adverse reactions between the two groups (P>0.05). CONCLUSION: Yinaojieyu prescription is effective in treating Parkinson's disease with kidney deficiency and liver depression type, and can improve the levels of DA, 5-HT and NE.

Effects of desflurane combined with dexmedetomidine in patients undergoing coronary artery bypass grafting 

LIU Ying, SHAO Junjin, SUN Daxin

2018, 23(4):  440-445.  doi:10.12092/j.issn.1009-2501.2018.04.013

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AIM: To investigate the effects of desflurane combined with dexmedetomidine on stress response, inflammatory response and myocardium in patients undergoing coronary artery bypass grafting (OPCABG).  METHODS: Sixty-eight patients undergoing OPCABG were randomly divided into observation group and control group according to the random number table method, with 34 cases in each group. Both groups were given midazolam 0.1-0.2 mg/kg, etomidate 0.15-0.3 mg/kg, sufentanil 2 μg/kg and vecuronium 0.1 mg/kg intravenous anesthesia induction. The observation group received intravenous dexmedetomidine 0.1-0.3 μg·kg^-1·h^-1 and desflurane with the lowest effective concentration (MAC) of 0.7-1.5, while the control group was given intravenous dexmedetomidine 0.5 μg·kg^-1·h^-1 to maintain anesthesia. The changes of cardiac index (CI), heart rate (HR), blood pressure (ABP), central venous pressure (CVP), pulmonary arterial pressure (PAP) and pulmonary arterial pressure (PAWP), plasma cTnI and the levels of creatine kinase isoenzyme（CK-MB）, COR, β-EP, CRP, IL-2 and interleukin 6 (IL-6) concentrations, adverse reactions and morphine use were compared between two groups. RESULTS: The CI of observation group at 0.5 h and1 h after bypass were significantly higher than that at 0.5 h after anesthesia; also, CI of observation group at 0.5 h was significantly higher than that of the control group; CI of control group at 1 h was significantly higher than that at 0.5 after anesthesia.HR in the observation group was significantly lower than that of the control group at 0.5 h after induction and HR was significantly higher at other time points than that at 0.5 h after anesthesia (P＜0.05). No significant difference was observed between other indicators (P＞0.05). Levels of cTn I and CK-MB in both groups continued to increase at 6, 12 and 24 h after operation, peaked at 24 h and decreased at 36 h after operation, both indicators were higher than those before operation; levels of cTnI and CK-MB at 12 h after operation in the observation group were significantly lower than those in the control group (P＜0.05).COR during the T1-4 period were significantly lower than that during T0 in the both groups; COR of the observation group during T2-4 period was significantly lower than that of the control group. β-EP and CRP during the T1-4 period were significantly lower than that during T0 in the both groups; β-EP and CRP of the observation group during T2-4 period was significantly lower than that of the control group. Levels of IL-2 in observation group were significantly higher than those in control group. Levels IL-6 during T1-4 in both groups were significantly lower than those during T0 period, and those of the observation group at T3 was significantly higher than that in control group (P＜0.05). No adverse reactions such as respiratory depression, bradycardia or hypotension occurred in both groups. Morphine usage in the control group was significantly higher than that in the observation group (χ²=4.570, P＜0.05). CONCLUSION: Desflurane combined with dexmedetomidine can be safely and effectively used in the OPCABG anesthesia process. It can effectively reduce the stress response and inflammatory reaction, reduce the myocardial injury and stabilize the hemodynamic status of patients, which is worthy of promotion.

Clinical study on the treatment of salvia ligustrazine injection on pregnant women with ICP in late pregnancy

YING Jiawei，WU Liang，SHENG Shaoqin

2018, 23(4):  446-450.  doi:10.12092/j.issn.1009-2501.2018.04.014

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 AIM: To investigate the clinical study on the effect of salvia ligustrazine injection on pregnant women with ICP in late pregnancy. METHODS: Two hundred and eighty-nine mild cases and one hundred and fifty-seven severe cases of pregnant women with ICP selected by ICP diagnosis and treatment guidelines (2015) were randomly divided into treatment group and control group. Mild control group received ursodeoxycholic acid 250 mg, oral, 3 times a day; severe control group received ursodeoxycholic acid 250 mg, oral, 3 times a day and S-adenosy-L-methionine 1.0 g, ivgtt, once a day. The treatment group was added with 5% glucose injection 500 mL+salvia ligustrazine injection 10 mL, ivgtt, once a day on the basis of the control group. Seven days counts for a course of treatment, the serum total bile acids (TBA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), human chorionic gonadotropin (hCG), S/D ratio of umbilical artery blood flow and pruritus scores were measured before and after treatment. RESULTS: After treatment, the levels of TBA, ALT, AST, hCG, S/D ratio and pruritus score in mild ICP were lower than those before treatment, there was statistically significant difference before and after treatment in both groups (P＜0.05),and there was no significant difference between the treatment group and the control group (P>0.05). After treatment, the levels of TBA, ALT, AST, hCG, S/D ratio and pruritus score in severe ICP were lower than those before treatment, there was statistically significant difference before and after treatment in both groups (P＜0.05), and there were significant differences between the treatment group and the control group (P＜0.05); there was a positive correlation between serum hCG and S/D ratio (in mild group r=0.498,0.479, P＜0.01;in severe group r=0.491,0.475, P＜0.01). CONCLUSION: Salvia miltiorrhiza injection can reduce the biochemical indexes, S/D ratio and hCG level of severe ICP pregnant women, and improve the symptoms of pruritus, but no obvious treatment effect on mild ICP pregnant women was observed.

Effects of Bushentongluo recipe combined with thermosensitive moxibustion therapy apparatus on lumbar disc herniation and its influence on inflammatory factors

SHENG Yougen, XU Haibo, XIA Jianhong, FANG Xing

2018, 23(4):  451-455.  doi:10.12092/j.issn.1009-2501.2018.04.015

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AIM: To investigate the effects of Bushentongluo recipe combined with Moxibustion with thermosensitive herbs on lumbar disc herniation and its influence on inflammatory factors.  METHODS: A total of 240 patients with lumbar disc herniation were divided into observation group and control group, 120 cases in each group. The control group was treated with thermosensitive moxibustion combined with tetrandrine. The observation group was treated with Bushentongluo recipe on the basis of the control group. The modified JOA spine function score (m-JOA scale) was used to assess the changes in the patient's condition. Visual analogue scale (VAS) was used to assess the patient's pain level. The quality of life was assessed by a health survey brief (SF-36 score). The levels of Creactionprotein, interleukin-1β, tumor necrosis factor-α and interleukin-6 were detected before and after treatment in the two groups.RESULTS:The curative effect of the observation group was better than that of the control group. The difference was statistically significant (P<0.05). The m-JOA scores and VAS scores of the two groups were significantly lower than those before treatment, and the SF-36 score was significantly higher than those before treatment (P<0.05). After treatment, the m-JOA score and VAS score of the observation group were significantly lower than that of the control group, and the SF-36 score was significantly higher than that of the control group. The difference was statistically significant (P<0.05). The levels of CRP, IL-1β, TNF-α and IL-6 in the two groups were significantly lower than those before treatment (P<0.05). After treatment, the CRP, IL-1β, TNF-α and IL-6 levels of the observation group were significantly lower than those of the control group. The difference was statistically significant (P<0.05). CONCLUSION: Bushentongluo recipe combined with Moxibustion with thermosensitive drugs is effective in treating lumbar disc herniation and can significantly reduce the inflammatory response.

Research progress of lncRNA and chemotherapy resistance in colorectal cancer

HU Yang, LI Zhixing, WANG Guo, ZHU Yuanshan

2018, 23(4):  456-463.  doi:10.12092/j.issn.1009-2501.2018.04.016

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Colorectal cancer (CRC) is a common gastrointestinal tumor with high incidence and mortality rates. Chemotherapy is the basic treatment for colorectal cancer, but chemoresistance is one of the most important causes of therapy failure in CRC, and the mechanism is not yet fully understood. Long noncoding RNA (lncRNA) represents a class of RNA transcripts longer than 200 nucleotides with no protein coding potential which could involve in cancer occurrence and development by the regulation of chromatin remodeling, transcription, and posttranscriptional processing. Recently, emerging evidences indicate that lncRNAs play an important role in the development and maintenance of chemoresistance. Different lncRNAs have been shown to confer chemoresistance in cancer cells by changing multiple drug resistance (MDR) transporters, modulating the cellular apoptosis rate, affecting the epithelial mesenchymal transition (EMT) process, and modulating signaling pathways. In this review, we discuss the mechanism of lncRNAs in drug resistance of colorectal cancer and provide a theoretical evidence for solving the problem of drug resistance in colorectal cancer.

ATP synthase beta subunit function and disease

YUAN Lijialong, QIN Xuping

2018, 23(4):  464-470.  doi:10.12092/j.issn.1009-2501.2018.04.017

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ATP synthase, also known as F1F0-ATP synthase, is the last link in the oxidative phosphorylation of respiratory chain in the mitochondrial membrane, chloroplast thylakoid membranes and photosynthetic bacteria membrane. The structure contains two subunits of F1 and F0, which are combined to decompose and synthesize ATP. ATP synthase beta subunit is an important component of F1F0-ATP synthase F1 subunit, which contains the catalytic sites needed for ATP synthesis and hydrolysis, and its function is directly related to the occurrence of disease. This article reviews the relationship between ATP synthase beta subunit and diabetes, tumor, obesity and other diseases.

Research progress of long noncoding RNA in systemic lupus erythematosus

CHEN Xinxin, CHEN Chaosheng

2018, 23(4):  471-476.  doi:10.12092/j.issn.1009-2501.2018.04.018

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Long noncoding RNAs (lncRNA), play significant role in regulation of epigenetics, chromatin remodeling, transcription and post transcriptional regulation and immune system regulation. Nonetheless, it once was seemed as "noise gene" among the complex modification of genetic functions. lncRNA is involved in the regulation of differentiation, activation and secretion of cytokines. In this study, we mainly focus on the role of lncRNA in the regulation of systemic lupus erythematosus.

Advances in the relationship between cytochrome P450 SNP and susceptibility to coronary heart disease

PENG Qiuju, YAN Huacheng, SHI Lei

2018, 23(4):  477-480.  doi:10.12092/j.issn.1009-2501.2018.04.019

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Coronary heart disease (CHD) is caused by genetic environmental interaction. Studies have shown that cytochrome P450 (CYP) metabolites such as epoxyeicosatrienoic acids (EETs), 20-hydroxy-eicosatetraenoic acid (20-HETE) and sex hormones play important roles in maintaining cardiovascular homeostasis. In vivo, CYP2C19 and CYP2J2 catalyze the metabolism of arachidonic acid (AA) to EETs; CYP4F2 and CYP1A1 are the main synthetases of 20-HETE; CYP17A1 and CYP19 are important synthetases of sex hormones. Therefore, polymorphism of the above CYP genes is likely to affect the development of CHD by influencing the production of its metabolites. This article reviews the five years' advances in the relationship between cytochrome P450 SNP and susceptibility to coronary heart disease so as to provide referential ideas for the personalized treatment of CHD.