Abstract:

AIM: To investigate the effects of immunoglobulin D (IgD) on the balance of Th1/Th2, Th17/Treg cells in healthy human T cell subsets.  METHODS: Different concentrations of IgD (final concentration of 1, 3,10 μg/mL) were added to stimulate the human peripheral mononuclear cells (PBMC). After 24 h, the expression of CD69 and CD154 and the percentage of Th1, Th2, Th17, and Treg subsets were detected by flow cytometry. The expression of nuclear transcription factors T-bet, GATA-3, Foxp3 and ROR-gammat mRNA was detected using qPCR assay. RESULTS: IgD could significantly increase the expression of CD69 and CD154（P<0.05）, which are markers of activate T cells. IgD could simultaneously decrease the percentage of Th1 and Treg subsets and significantly increased the percentage of Th17 subset, but had no significant effect on Th2 subset. IgD significantly inhibited the expression of T-bet and Foxp3 mRNA and significantly promoted the expression of ROR-gammat mRNA, but had no significant effect on the expression of GATA-3 mRNA. CONCLUSION: IgD can activate T cells and induce the imbalance between Th1/Th2 and Th17/Treg subsets.

Key words: immunoglobulin D, T cell subset, nuclear transcription factor, expression, imbalance