Protective effects of manganese superoxide dismutase mimic on liver injury in mice induced by carbon tetrachloride

doi:10.12092/j.issn.1009-2501.2018.11.002

Abstract

Abstract:

AIM: To investigate the effects of manganese superoxide dismutase mimic (MnSODm) on liver function and liver histological morphology in mice and to probe into its underlying mechanism. METHODS: Sixty mice were randomized into normal group, model group, bifendate group (50 mg/kg) and different doses of MnSODm (10, 20 and 40 mg/kg) group. Liver injury was induced by carbon tetrachloride (CCl₄) in mice. The serum and liver activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. Liver tissue sections were examined under a light microscope. The activities of superoxide dismutase (SOD) and catcalase (CAT) and the level of glutathione (GSH) in liver homogenate were also detected. The contents of IL-1β, IFN-γ in liver homogenate were measured by ELISA. RESULTS: In the model of acute liver injury induced by CCl₄in mice, MnSODm markedly decreased the elevated serum and liver activities of ALT and AST. MnSODm effectively improved the tissue lesion of liver. In addition, MnSODm significantly increased SOD activity and GSH level (P<0.05 or P<0.01), and decreased activity of CAT and content of IL-1β, IFN-γ in liver (P<0.05 or P<0.01). CONCLUSION: The MnSODm has protective effect on acute liver injury induced by CCl4 in mice. The mechanism may be related to inhibition of oxidative stress and inflammatory reaction.

Key words: manganese superoxide dismutase mimic, carbon tetrachloride, liver injury, protective effects

CLC Number:

WANG Yanhong, FENG Tiexin, PU Junfeng, ZHANG Lingfang, LI Hongling. Protective effects of manganese superoxide dismutase mimic on liver injury in mice induced by carbon tetrachloride[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(11): 1209-1214.

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